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dc.contributor.authorGentzel, Men_US
dc.contributor.authorPardo, Men_US
dc.contributor.authorSubramaniam, Sen_US
dc.contributor.authorStewart, AFen_US
dc.contributor.authorChoudhary, JSen_US
dc.date.accessioned2019-06-04T09:57:48Z
dc.date.issued2019-07en_US
dc.identifier.citationMethods (San Diego, Calif.), 2019, 164-165 pp. 67 - 72en_US
dc.identifier.issn1046-2023en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3249
dc.identifier.eissn1095-9130en_US
dc.identifier.doi10.1016/j.ymeth.2019.03.028en_US
dc.description.abstractThe identification of bona fide protein-protein interactions and the mapping of proteomes was greatly enhanced by protein tagging for generic affinity purification methods and analysis by mass spectrometry (AP-MS). The high quality of AP-MS data permitted the development of proteomic navigation by sequential tagging of identified interactions. However AP-MS is laborious and limited to relatively high affinity protein-protein interactions. Proximity labeling, first with the biotin ligase BirA, termed BioID, and then with ascorbate peroxidase, termed APEX, permits a greater reach into the proteome than AP-MS enabling both the identification of a wider field and weaker protein-protein interactions. This additional reach comes with the need for stringent controls. Proximity labeling also permits experiments in living cells allowing spatiotemporal investigations of the proteome. Here we discuss proximity labeling with accompanying methodological descriptions for E. coli and mammalian cells.en_US
dc.formatPrint-Electronicen_US
dc.format.extent67 - 72en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_US
dc.titleProteomic navigation using proximity-labeling.en_US
dc.typeJournal Article
dcterms.dateAccepted2019-03-29en_US
rioxxterms.versionofrecord10.1016/j.ymeth.2019.03.028en_US
rioxxterms.licenseref.startdate2019-07en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfMethods (San Diego, Calif.)en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Functional Proteomics Group
pubs.publication-statusPublisheden_US
pubs.volume164-165en_US
pubs.embargo.termsNot knownen_US
icr.researchteamFunctional Proteomics Groupen_US
dc.contributor.icrauthorPardo Calvo, Maria Mercedesen_US
dc.contributor.icrauthorChoudhary, Jyotien_US


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