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dc.contributor.authorStowe, CL
dc.contributor.authorBurley, TA
dc.contributor.authorAllan, H
dc.contributor.authorVinci, M
dc.contributor.authorKramer-Marek, G
dc.contributor.authorCiobota, DM
dc.contributor.authorParkinson, GN
dc.contributor.authorSouthworth, TL
dc.contributor.authorAgliardi, G
dc.contributor.authorHotblack, A
dc.contributor.authorLythgoe, MF
dc.contributor.authorBranchini, BR
dc.contributor.authorKalber, TL
dc.contributor.authorAnderson, JC
dc.contributor.authorPule, MA
dc.date.accessioned2019-10-23T10:18:40Z
dc.date.issued2019-10-15
dc.identifier.citationeLife, 2019, 8
dc.identifier.issn2050-084X
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3391
dc.identifier.eissn2050-084X
dc.identifier.doi10.7554/elife.45801
dc.description.abstractBioluminescence imaging (BLI) is ubiquitous in scientific research for the sensitive tracking of biological processes in small animal models. However, due to the attenuation of visible light by tissue, and the limited set of near-infrared bioluminescent enzymes, BLI is largely restricted to monitoring single processes in vivo. Here we show, that by combining stabilised colour mutants of firefly luciferase (FLuc) with the luciferin (LH2) analogue infraluciferin (iLH2), near-infrared dual BLI can be achieved in vivo. The X-ray crystal structure of FLuc with a high-energy intermediate analogue, 5'-O-[N-(dehydroinfraluciferyl)sulfamoyl] adenosine (iDLSA) provides insight into the FLuc-iLH2 reaction leading to near-infrared light emission. The spectral characterisation and unmixing validation studies reported here established that iLH2 is superior to LH2 for the spectral unmixing of bioluminescent signals in vivo; which led to this novel near-infrared dual BLI system being applied to monitor both tumour burden and CAR T cell therapy within a systemically induced mouse tumour model.
dc.formatElectronic
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectAnimals
dc.subjectMice
dc.subjectNeoplasms
dc.subjectDisease Models, Animal
dc.subjectLuminescent Proteins
dc.subjectRecombinant Fusion Proteins
dc.subjectCrystallography, X-Ray
dc.subjectLuminescent Measurements
dc.subjectNeoplasm Transplantation
dc.subjectProtein Conformation
dc.subjectMale
dc.subjectOptical Imaging
dc.titleNear-infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin.
dc.typeJournal Article
dcterms.dateAccepted2019-09-25
rioxxterms.versionofrecord10.7554/elife.45801
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2019-10-15
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfeLife
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Therapeutics
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Preclinical Molecular Imaging
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Preclinical Molecular Imaging
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Therapeutics
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Preclinical Molecular Imaging
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Preclinical Molecular Imaging
pubs.publication-statusPublished
pubs.volume8en_US
pubs.embargo.termsNot known
icr.researchteamPreclinical Molecular Imagingen_US
dc.contributor.icrauthorKramer-Marek, Gabrielaen


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