dc.contributor.author | Stowe, CL | |
dc.contributor.author | Burley, TA | |
dc.contributor.author | Allan, H | |
dc.contributor.author | Vinci, M | |
dc.contributor.author | Kramer-Marek, G | |
dc.contributor.author | Ciobota, DM | |
dc.contributor.author | Parkinson, GN | |
dc.contributor.author | Southworth, TL | |
dc.contributor.author | Agliardi, G | |
dc.contributor.author | Hotblack, A | |
dc.contributor.author | Lythgoe, MF | |
dc.contributor.author | Branchini, BR | |
dc.contributor.author | Kalber, TL | |
dc.contributor.author | Anderson, JC | |
dc.contributor.author | Pule, MA | |
dc.date.accessioned | 2019-10-23T10:18:40Z | |
dc.date.issued | 2019-10-15 | |
dc.identifier.citation | eLife, 2019, 8 | |
dc.identifier.issn | 2050-084X | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/3391 | |
dc.identifier.eissn | 2050-084X | |
dc.identifier.doi | 10.7554/elife.45801 | |
dc.description.abstract | Bioluminescence imaging (BLI) is ubiquitous in scientific research for the sensitive tracking of biological processes in small animal models. However, due to the attenuation of visible light by tissue, and the limited set of near-infrared bioluminescent enzymes, BLI is largely restricted to monitoring single processes in vivo. Here we show, that by combining stabilised colour mutants of firefly luciferase (FLuc) with the luciferin (LH2) analogue infraluciferin (iLH2), near-infrared dual BLI can be achieved in vivo. The X-ray crystal structure of FLuc with a high-energy intermediate analogue, 5'-O-[N-(dehydroinfraluciferyl)sulfamoyl] adenosine (iDLSA) provides insight into the FLuc-iLH2 reaction leading to near-infrared light emission. The spectral characterisation and unmixing validation studies reported here established that iLH2 is superior to LH2 for the spectral unmixing of bioluminescent signals in vivo; which led to this novel near-infrared dual BLI system being applied to monitor both tumour burden and CAR T cell therapy within a systemically induced mouse tumour model. | |
dc.format | Electronic | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | eLIFE SCIENCES PUBL LTD | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Animals | |
dc.subject | Mice | |
dc.subject | Neoplasms | |
dc.subject | Disease Models, Animal | |
dc.subject | Luminescent Proteins | |
dc.subject | Recombinant Fusion Proteins | |
dc.subject | Crystallography, X-Ray | |
dc.subject | Luminescent Measurements | |
dc.subject | Neoplasm Transplantation | |
dc.subject | Protein Conformation | |
dc.subject | Male | |
dc.subject | Optical Imaging | |
dc.title | Near-infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2019-09-25 | |
rioxxterms.versionofrecord | 10.7554/elife.45801 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2019-10-15 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | eLife | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Preclinical Molecular Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Preclinical Molecular Imaging | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Preclinical Molecular Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Preclinical Molecular Imaging | |
pubs.publication-status | Published | |
pubs.volume | 8 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Preclinical Molecular Imaging | |
dc.contributor.icrauthor | Kramer-Marek, Gabriela | |