Near-infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin.
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Date
2019-10-15ICR Author
Author
Stowe, CL
Burley, TA
Allan, H
Vinci, M
Kramer-Marek, G
Ciobota, DM
Parkinson, GN
Southworth, TL
Agliardi, G
Hotblack, A
Lythgoe, MF
Branchini, BR
Kalber, TL
Anderson, JC
Pule, MA
Type
Journal Article
Metadata
Show full item recordAbstract
Bioluminescence imaging (BLI) is ubiquitous in scientific research for the sensitive tracking of biological processes in small animal models. However, due to the attenuation of visible light by tissue, and the limited set of near-infrared bioluminescent enzymes, BLI is largely restricted to monitoring single processes in vivo. Here we show, that by combining stabilised colour mutants of firefly luciferase (FLuc) with the luciferin (LH2) analogue infraluciferin (iLH2), near-infrared dual BLI can be achieved in vivo. The X-ray crystal structure of FLuc with a high-energy intermediate analogue, 5'-O-[N-(dehydroinfraluciferyl)sulfamoyl] adenosine (iDLSA) provides insight into the FLuc-iLH2 reaction leading to near-infrared light emission. The spectral characterisation and unmixing validation studies reported here established that iLH2 is superior to LH2 for the spectral unmixing of bioluminescent signals in vivo; which led to this novel near-infrared dual BLI system being applied to monitor both tumour burden and CAR T cell therapy within a systemically induced mouse tumour model.
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Subject
Animals
Mice
Neoplasms
Disease Models, Animal
Luminescent Proteins
Recombinant Fusion Proteins
Crystallography, X-Ray
Luminescent Measurements
Neoplasm Transplantation
Protein Conformation
Male
Optical Imaging
Research team
Preclinical Molecular Imaging
Language
eng
Date accepted
2019-09-25
License start date
2019-10-15
Citation
eLife, 2019, 8
Publisher
eLIFE SCIENCES PUBL LTD