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dc.contributor.authorMartinez, VG
dc.contributor.authorPankova, V
dc.contributor.authorKrasny, L
dc.contributor.authorSingh, T
dc.contributor.authorMakris, S
dc.contributor.authorWhite, IJ
dc.contributor.authorBenjamin, AC
dc.contributor.authorDertschnig, S
dc.contributor.authorHorsnell, HL
dc.contributor.authorKriston-Vizi, J
dc.contributor.authorBurden, JJ
dc.contributor.authorHuang, PH
dc.contributor.authorTape, CJ
dc.contributor.authorActon, SE
dc.date.accessioned2019-12-06T14:32:48Z
dc.date.issued2019-11
dc.identifier.citationCell reports, 2019, 29 (9), pp. 2810 - 2822.e5
dc.identifier.issn2211-1247
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3442
dc.identifier.eissn2211-1247
dc.identifier.doi10.1016/j.celrep.2019.10.103
dc.description.abstractLymph nodes (LNs) act as filters, constantly sampling peripheral cues. This is facilitated by the conduit network, a tubular structure of aligned extracellular matrix (ECM) fibrils ensheathed by fibroblastic reticular cells (FRCs). LNs undergo rapid 3- to 5-fold expansion during adaptive immune responses, but these ECM-rich structures are not permanently damaged. Whether conduit flow or filtering function is affected during LN expansion is unknown. Here, we show that conduits are partially disrupted during acute LN expansion, but FRC-FRC contacts remain connected. We reveal that polarized FRCs deposit ECM basolaterally using LL5-β and that ECM production is regulated at transcriptional and secretory levels by the C-type lectin CLEC-2, expressed by dendritic cells. Inflamed LNs maintain conduit size exclusion, and flow is disrupted but persists, indicating the robustness of this structure despite rapid tissue expansion. We show how dynamic communication between peripheral tissues and LNs provides a mechanism to prevent inflammation-induced fibrosis in lymphoid tissue.
dc.formatPrint
dc.format.extent2810 - 2822.e5
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectLymph Nodes
dc.subjectExtracellular Matrix
dc.subjectFibroblasts
dc.titleFibroblastic Reticular Cells Control Conduit Matrix Deposition during Lymph Node Expansion.
dc.typeJournal Article
dcterms.dateAccepted2019-10-25
rioxxterms.versionofrecord10.1016/j.celrep.2019.10.103
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2019-11
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfCell reports
pubs.issue9
pubs.notesNo embargo
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Oncogene
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Molecular and Systems Oncology
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Oncogene
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Molecular and Systems Oncology
pubs.publication-statusPublished
pubs.volume29
pubs.embargo.termsNo embargo
icr.researchteamOncogeneen_US
icr.researchteamMolecular and Systems Oncologyen_US
dc.contributor.icrauthorTape, Christopheren
dc.contributor.icrauthorKrasny, Lukasen
dc.contributor.icrauthorHuang, Paulen


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