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dc.contributor.authorZhang, W
dc.contributor.authorChronis, C
dc.contributor.authorChen, X
dc.contributor.authorZhang, H
dc.contributor.authorSpalinskas, R
dc.contributor.authorPardo, M
dc.contributor.authorChen, L
dc.contributor.authorWu, G
dc.contributor.authorZhu, Z
dc.contributor.authorYu, Y
dc.contributor.authorYu, L
dc.contributor.authorChoudhary, J
dc.contributor.authorNichols, J
dc.contributor.authorParast, MM
dc.contributor.authorGreber, B
dc.contributor.authorSahlén, P
dc.contributor.authorPlath, K
dc.date.accessioned2020-06-11T12:18:36Z
dc.date.issued2019-01
dc.identifier.citationCell stem cell, 2019, 24 (1), pp. 138 - 152.e8
dc.identifier.issn1934-5909
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3726
dc.identifier.eissn1875-9777
dc.identifier.doi10.1016/j.stem.2018.12.001
dc.description.abstractBAF complexes are composed of different subunits with varying functional and developmental roles, although many subunits have not been examined in depth. Here we show that the Baf45 subunit Dpf2 maintains pluripotency and ESC differentiation potential. Dpf2 co-occupies enhancers with Oct4, Sox2, p300, and the BAF subunit Brg1, and deleting Dpf2 perturbs ESC self-renewal, induces repression of Tbx3, and impairs mesendodermal differentiation without dramatically altering Brg1 localization. Mesendodermal differentiation can be rescued by restoring Tbx3 expression, whose distal enhancer is positively regulated by Dpf2-dependent H3K27ac maintenance and recruitment of pluripotency TFs and Brg1. In contrast, the PRC2 subunit Eed binds an intragenic Tbx3 enhancer to oppose Dpf2-dependent Tbx3 expression and mesendodermal differentiation. The PRC2 subunit Ezh2 likewise opposes Dpf2-dependent differentiation through a distinct mechanism involving Nanog repression. Together, these findings delineate distinct mechanistic roles for specific BAF and PRC2 subunits during ESC differentiation.
dc.formatPrint
dc.format.extent138 - 152.e8
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectAnimals
dc.subjectMice, Knockout
dc.subjectMice
dc.subjectDNA-Binding Proteins
dc.subjectT-Box Domain Proteins
dc.subjectHistones
dc.subjectProtein Subunits
dc.subjectTranscription Factors
dc.subjectCell Cycle
dc.subjectApoptosis
dc.subjectCell Differentiation
dc.subjectEmbryonic Stem Cells
dc.subjectPolycomb Repressive Complex 2
dc.subjectNanog Homeobox Protein
dc.titleThe BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation.
dc.typeJournal Article
dcterms.dateAccepted2018-12-05
rioxxterms.versionofrecord10.1016/j.stem.2018.12.001
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2019-01
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfCell stem cell
pubs.issue1
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Functional Proteomics Group
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Functional Proteomics Group
pubs.publication-statusPublished
pubs.volume24
pubs.embargo.termsNot known
icr.researchteamFunctional Proteomics Groupen_US
dc.contributor.icrauthorPardo Calvo, Maria Mercedesen
dc.contributor.icrauthorChoudhary, Jyotien


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