Show simple item record

dc.contributor.authorAlatwi, HEen_US
dc.contributor.authorDowns, JAen_US
dc.date.accessioned2020-07-24T15:04:26Z
dc.date.issued2015-08en_US
dc.identifier.citationEMBO reports, 2015, 16 (8), pp. 986 - 994en_US
dc.identifier.issn1469-221Xen_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3868
dc.identifier.eissn1469-3178en_US
dc.identifier.doi10.15252/embr.201540330en_US
dc.description.abstractThe mammalian INO80 remodelling complex facilitates homologous recombination (HR), but the mechanism by which it does this is unclear. Budding yeast INO80 can remove H2A.Z/H2B dimers from chromatin and replace them with H2A/H2B dimers. H2A.Z is actively incorporated at sites of damage in mammalian cells, raising the possibility that H2A.Z may need to be subsequently removed for resolution of repair. Here, we show that H2A.Z in human cells is indeed rapidly removed from chromatin flanking DNA damage by INO80. We also report that the histone chaperone ANP32E, which is implicated in removing H2AZ from chromatin, similarly promotes HR and appears to work on the same pathway as INO80 in these assays. Importantly, we demonstrate that the HR defect in cells depleted of INO80 or ANP32E can be rescued by H2A.Z co-depletion, suggesting that H2A.Z removal from chromatin is the primary function of INO80 and ANP32E in promoting homologous recombination.en_US
dc.formatPrint-Electronicen_US
dc.format.extent986 - 994en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectCell Line, Tumoren_US
dc.subjectHela Cellsen_US
dc.subjectChromatinen_US
dc.subjectHumansen_US
dc.subjectDNA Damageen_US
dc.subjectDNA Helicasesen_US
dc.subjectMolecular Chaperonesen_US
dc.subjectNuclear Proteinsen_US
dc.subjectHistonesen_US
dc.subjectPhosphoproteinsen_US
dc.subjectRNA, Small Interferingen_US
dc.subjectChromatin Assembly and Disassemblyen_US
dc.subjectDNA Repairen_US
dc.subjectGene Knockdown Techniquesen_US
dc.subjectHomologous Recombinationen_US
dc.titleRemoval of H2A.Z by INO80 promotes homologous recombination.en_US
dc.typeJournal Article
dcterms.dateAccepted2015-06-10en_US
rioxxterms.versionofrecord10.15252/embr.201540330en_US
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0en_US
rioxxterms.licenseref.startdate2015-08en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfEMBO reportsen_US
pubs.issue8en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Epigenetics and Genome Stability
pubs.publication-statusPublisheden_US
pubs.volume16en_US
pubs.embargo.termsNot knownen_US
icr.researchteamEpigenetics and Genome Stabilityen_US
dc.contributor.icrauthorDowns, Jessicaen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

http://creativecommons.org/licenses/by/4.0/
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/