dc.contributor.author | Alatwi, HE | |
dc.contributor.author | Downs, JA | |
dc.date.accessioned | 2020-07-24T15:04:26Z | |
dc.date.issued | 2015-08-01 | |
dc.identifier.citation | EMBO reports, 2015, 16 (8), pp. 986 - 994 | |
dc.identifier.issn | 1469-221X | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/3868 | |
dc.identifier.eissn | 1469-3178 | |
dc.identifier.doi | 10.15252/embr.201540330 | |
dc.description.abstract | The mammalian INO80 remodelling complex facilitates homologous recombination (HR), but the mechanism by which it does this is unclear. Budding yeast INO80 can remove H2A.Z/H2B dimers from chromatin and replace them with H2A/H2B dimers. H2A.Z is actively incorporated at sites of damage in mammalian cells, raising the possibility that H2A.Z may need to be subsequently removed for resolution of repair. Here, we show that H2A.Z in human cells is indeed rapidly removed from chromatin flanking DNA damage by INO80. We also report that the histone chaperone ANP32E, which is implicated in removing H2AZ from chromatin, similarly promotes HR and appears to work on the same pathway as INO80 in these assays. Importantly, we demonstrate that the HR defect in cells depleted of INO80 or ANP32E can be rescued by H2A.Z co-depletion, suggesting that H2A.Z removal from chromatin is the primary function of INO80 and ANP32E in promoting homologous recombination. | |
dc.format | Print-Electronic | |
dc.format.extent | 986 - 994 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | WILEY | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Cell Line, Tumor | |
dc.subject | Hela Cells | |
dc.subject | Chromatin | |
dc.subject | Humans | |
dc.subject | DNA Damage | |
dc.subject | DNA Helicases | |
dc.subject | Molecular Chaperones | |
dc.subject | Nuclear Proteins | |
dc.subject | Histones | |
dc.subject | Phosphoproteins | |
dc.subject | RNA, Small Interfering | |
dc.subject | Chromatin Assembly and Disassembly | |
dc.subject | DNA Repair | |
dc.subject | Gene Knockdown Techniques | |
dc.subject | Homologous Recombination | |
dc.title | Removal of H2A.Z by INO80 promotes homologous recombination. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2015-06-10 | |
rioxxterms.versionofrecord | 10.15252/embr.201540330 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2015-08 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | EMBO reports | |
pubs.issue | 8 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Epigenetics and Genome Stability | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Epigenetics and Genome Stability | |
pubs.publication-status | Published | |
pubs.volume | 16 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Epigenetics and Genome Stability | |
dc.contributor.icrauthor | Downs, Jessica | |