Show simple item record

dc.contributor.authorSero, JEen_US
dc.contributor.authorSailem, HZen_US
dc.contributor.authorArdy, RCen_US
dc.contributor.authorAlmuttaqi, Hen_US
dc.contributor.authorZhang, Ten_US
dc.contributor.authorBakal, Cen_US
dc.date.accessioned2020-07-28T14:11:07Z
dc.date.issued2015-03en_US
dc.identifier.citationMolecular systems biology, 2015, 11 (3), pp. 790 - ?en_US
dc.identifier.issn1744-4292en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3887
dc.identifier.eissn1744-4292en_US
dc.identifier.doi10.15252/msb.20145644en_US
dc.description.abstractAlthough a great deal is known about the signaling events that promote nuclear translocation of NF-κB, how cellular biophysics and the microenvironment might regulate the dynamics of this pathway is poorly understood. In this study, we used high-content image analysis and Bayesian network modeling to ask whether cell shape and context features influence NF-κB activation using the inherent variability present in unperturbed populations of breast tumor and non-tumor cell lines. Cell–cell contact, cell and nuclear area, and protrusiveness all contributed to variability in NF-κB localization in the absence and presence of TNFα. Higher levels of nuclear NF-κB were associated with mesenchymal-like versus epithelial-like morphologies, and RhoA-ROCK-myosin II signaling was critical for mediating shape-based differences in NF-κB localization and oscillations. Thus, mechanical factors such as cell shape and the microenvironment can influence NF-κB signaling and may in part explain how different phenotypic outcomes can arise from the same chemical cues.en_US
dc.formatPrinten_US
dc.format.extent790 - ?en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectBreasten_US
dc.subjectCell Lineen_US
dc.subjectCell Nucleusen_US
dc.subjectEpithelial Cellsen_US
dc.subjectHumansen_US
dc.subjectNF-kappa Ben_US
dc.subjectBayes Theoremen_US
dc.subjectSignal Transductionen_US
dc.subjectCell Shapeen_US
dc.subjectProtein Transporten_US
dc.subjectFemaleen_US
dc.subjectCellular Microenvironmenten_US
dc.subjectMCF-7 Cellsen_US
dc.titleCell shape and the microenvironment regulate nuclear translocation of NF-κB in breast epithelial and tumor cells.en_US
dc.typeJournal Article
rioxxterms.versionofrecord10.15252/msb.20145644en_US
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0en_US
rioxxterms.licenseref.startdate2015-03en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfMolecular systems biologyen_US
pubs.issue3en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Dynamical Cell Systems
pubs.publication-statusPublisheden_US
pubs.volume11en_US
pubs.embargo.termsNot knownen_US
icr.researchteamDynamical Cell Systemsen_US
dc.contributor.icrauthorBakal, Christopheren_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

http://creativecommons.org/licenses/by/4.0/
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/