dc.contributor.author | Sero, JE | |
dc.contributor.author | Sailem, HZ | |
dc.contributor.author | Ardy, RC | |
dc.contributor.author | Almuttaqi, H | |
dc.contributor.author | Zhang, T | |
dc.contributor.author | Bakal, C | |
dc.date.accessioned | 2020-07-28T14:11:07Z | |
dc.date.issued | 2015-03-03 | |
dc.identifier.citation | Molecular systems biology, 2015, 11 (3), pp. 790 - ? | |
dc.identifier.issn | 1744-4292 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/3887 | |
dc.identifier.eissn | 1744-4292 | |
dc.identifier.doi | 10.15252/msb.20145644 | |
dc.description.abstract | Although a great deal is known about the signaling events that promote nuclear translocation of NF-κB, how cellular biophysics and the microenvironment might regulate the dynamics of this pathway is poorly understood. In this study, we used high-content image analysis and Bayesian network modeling to ask whether cell shape and context features influence NF-κB activation using the inherent variability present in unperturbed populations of breast tumor and non-tumor cell lines. Cell–cell contact, cell and nuclear area, and protrusiveness all contributed to variability in NF-κB localization in the absence and presence of TNFα. Higher levels of nuclear NF-κB were associated with mesenchymal-like versus epithelial-like morphologies, and RhoA-ROCK-myosin II signaling was critical for mediating shape-based differences in NF-κB localization and oscillations. Thus, mechanical factors such as cell shape and the microenvironment can influence NF-κB signaling and may in part explain how different phenotypic outcomes can arise from the same chemical cues. | |
dc.format | Print | |
dc.format.extent | 790 - ? | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | WILEY | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Breast | |
dc.subject | Cell Line | |
dc.subject | Cell Nucleus | |
dc.subject | Epithelial Cells | |
dc.subject | Humans | |
dc.subject | NF-kappa B | |
dc.subject | Bayes Theorem | |
dc.subject | Signal Transduction | |
dc.subject | Cell Shape | |
dc.subject | Protein Transport | |
dc.subject | Female | |
dc.subject | Cellular Microenvironment | |
dc.subject | MCF-7 Cells | |
dc.title | Cell shape and the microenvironment regulate nuclear translocation of NF-κB in breast epithelial and tumor cells. | |
dc.type | Journal Article | |
rioxxterms.versionofrecord | 10.15252/msb.20145644 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2015-03 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Molecular systems biology | |
pubs.issue | 3 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Dynamical Cell Systems | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Dynamical Cell Systems | |
pubs.publication-status | Published | |
pubs.volume | 11 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Dynamical Cell Systems | |
dc.contributor.icrauthor | Bakal, Christopher | |