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dc.contributor.authorGrant, R
dc.contributor.authorAbdelbaki, A
dc.contributor.authorBertoldi, A
dc.contributor.authorGavilan, MP
dc.contributor.authorMansfeld, J
dc.contributor.authorGlover, DM
dc.contributor.authorLindon, C
dc.date.accessioned2020-08-26T13:25:24Z
dc.date.issued2018-06
dc.identifier.citationOpen biology, 2018, 8 (6)
dc.identifier.issn2046-2441
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4015
dc.identifier.eissn2046-2441
dc.identifier.doi10.1098/rsob.170272
dc.description.abstractAurora A kinase (AURKA) is a major regulator of mitosis and an important driver of cancer progression. The roles of AURKA outside of mitosis, and how these might contribute to cancer progression, are not well understood. Here, we show that a fraction of cytoplasmic AURKA is associated with mitochondria, co-fractionating in cell extracts and interacting with mitochondrial proteins by reciprocal co-immunoprecipitation. We have also found that the dynamics of the mitochondrial network are sensitive to AURKA inhibition, depletion or overexpression. This can account for the different mitochondrial morphologies observed in RPE-1 and U2OS cell lines, which show very different levels of expression of AURKA. We identify the mitochondrial fraction of AURKA as influencing mitochondrial morphology, because an N-terminally truncated version of the kinase that does not localize to mitochondria does not affect the mitochondrial network. We identify a cryptic mitochondrial targeting sequence in the AURKA N-terminus and discuss how alternative conformations of the protein may influence its cytoplasmic fate.
dc.formatPrint
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectCell Line
dc.subjectCytoplasm
dc.subjectMitochondria
dc.subjectHumans
dc.subjectMitochondrial Proteins
dc.subjectProtein Kinase Inhibitors
dc.subjectProteomics
dc.subjectProtein Binding
dc.subjectAurora Kinase A
dc.titleConstitutive regulation of mitochondrial morphology by Aurora A kinase depends on a predicted cryptic targeting sequence at the N-terminus.
dc.typeJournal Article
dcterms.dateAccepted2018-05-18
rioxxterms.versionofrecord10.1098/rsob.170272
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2018-06
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfOpen biology
pubs.issue6
pubs.notesNo embargo
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Post-translational modifications and cell proliferation
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Post-translational modifications and cell proliferation
pubs.publication-statusPublished
pubs.volume8en_US
pubs.embargo.termsNo embargo
icr.researchteamPost-translational modifications and cell proliferationen_US
dc.contributor.icrauthorMansfeld, Joergen


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