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dc.contributor.authorBarreiro-Alonso, A
dc.contributor.authorLamas-Maceiras, M
dc.contributor.authorGarcía-Díaz, R
dc.contributor.authorRodríguez-Belmonte, E
dc.contributor.authorYu, L
dc.contributor.authorPardo, M
dc.contributor.authorChoudhary, JS
dc.contributor.authorCerdán, ME
dc.date.accessioned2020-10-15T15:16:43Z
dc.date.issued2018-04-10
dc.identifier.citationOncotarget, 2018, 9 (27), pp. 19050 - 19064
dc.identifier.issn1949-2553
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4154
dc.identifier.eissn1949-2553
dc.identifier.doi10.18632/oncotarget.24887
dc.description.abstractHigh Mobility Group B (HMGB) proteins are involved in cancer progression and in cellular responses to platinum compounds used in the chemotherapy of prostate and ovary cancer. Here we use affinity purification coupled to mass spectrometry (MS) and yeast two-hybrid (Y2H) screening to carry out an exhaustive study of HMGB1 and HMGB2 protein interactions in the context of prostate and ovary epithelia. We present a proteomic study of HMGB1 partners based on immunoprecipitation of HMGB1 from a non-cancerous prostate epithelial cell line. In addition, HMGB1 and HMGB2 were used as baits in yeast two-hybrid screening of libraries from prostate and ovary epithelial cell lines as well as from healthy ovary tissue. HMGB1 interacts with many nuclear proteins that control gene expression, but also with proteins that form part of the cytoskeleton, cell-adhesion structures and others involved in intracellular protein translocation, cellular migration, secretion, apoptosis and cell survival. HMGB2 interacts with proteins involved in apoptosis, cell motility and cellular proliferation. High confidence interactors, based on repeated identification in different cell types or in both MS and Y2H approaches, are discussed in relation to cancer. This study represents a useful resource for detailed investigation of the role of HMGB1 in cancer of epithelial origins, as well as potential alternative avenues of therapeutic intervention.
dc.formatElectronic-eCollection
dc.format.extent19050 - 19064
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleDelineating the HMGB1 and HMGB2 interactome in prostate and ovary epithelial cells and its relationship with cancer.
dc.typeJournal Article
dcterms.dateAccepted2018-02-27
rioxxterms.versionofrecord10.18632/oncotarget.24887
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2018-04-10
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfOncotarget
pubs.issue27
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Functional Proteomics Group
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Functional Proteomics Group
pubs.publication-statusPublished
pubs.volume9
pubs.embargo.termsNot known
icr.researchteamFunctional Proteomics Groupen_US
dc.contributor.icrauthorPardo Calvo, Maria Mercedesen
dc.contributor.icrauthorChoudhary, Jyotien


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