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dc.contributor.authorStevenson, J
dc.contributor.authorBarrow-McGee, R
dc.contributor.authorYu, L
dc.contributor.authorPaul, A
dc.contributor.authorMansfield, D
dc.contributor.authorOwen, J
dc.contributor.authorWoodman, N
dc.contributor.authorNatrajan, R
dc.contributor.authorHaider, S
dc.contributor.authorGillett, C
dc.contributor.authorTutt, A
dc.contributor.authorPinder, SE
dc.contributor.authorChoudary, J
dc.contributor.authorNaidoo, K
dc.date.accessioned2021-03-31T10:16:06Z
dc.date.available2021-03-31T10:16:06Z
dc.date.issued2021-03-05
dc.identifier.citationNPJ breast cancer, 2021, 7 (1), pp. 24 - ?en_US
dc.identifier.issn2374-4677
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4474
dc.identifier.eissn2374-4677en_US
dc.identifier.eissn2374-4677
dc.identifier.doi10.1038/s41523-021-00227-7en_US
dc.identifier.doi10.1038/s41523-021-00227-7
dc.description.abstractIn breast cancer (BC), detecting low volumes of axillary lymph node (ALN) metastasis pre-operatively is difficult and novel biomarkers are needed. We recently showed that patient-derived ALNs can be sustained ex-vivo using normothermic perfusion. We now compare reactive (tumour-free; n = 5) and macrometastatic (containing tumour deposits >2 mm; n = 4) ALNs by combining whole section multiplex immunofluorescence with TMT-labelled LC-MS/MS of the circulating perfusate. Macrometastases contained significantly fewer B cells and T cells (CD4<sup>+</sup>/CD8<sup>+</sup>/regulatory) than reactive nodes (p = 0.02). Similarly, pathway analysis of the perfusate proteome (119/1453 proteins significantly differentially expressed) showed that immune function was diminished in macrometastases in favour of 'extracellular matrix degradation'; only 'neutrophil degranulation' was preserved. Qualitative comparison of the perfusate proteome to that of node-positive pancreatic and prostatic adenocarcinoma also highlighted 'neutrophil degranulation' as a contributing factor to nodal metastasis. Thus, metastasis-induced changes in the REPLICANT perfusate proteome are detectable, and could facilitate biomarker discovery.en_US
dc.formatElectronicen_US
dc.format.extent24 - ?en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.titleProteomics of REPLICANT perfusate detects changes in the metastatic lymph node microenvironment.en_US
dc.typeJournal Article
dcterms.dateAccepted2021-01-20
rioxxterms.versionVoRen_US
rioxxterms.versionofrecord10.1038/s41523-021-00227-7en_US
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0en_US
rioxxterms.licenseref.startdate2021-03-05
dc.relation.isPartOfNPJ breast canceren_US
pubs.issue1en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research/Functional Genomics
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Functional Genomics
pubs.publication-statusPublisheden_US
pubs.volume7en_US
pubs.embargo.termsNot knownen_US
icr.researchteamFunctional Genomics
dc.contributor.icrauthorNatrajan, Rachaelen_US


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