dc.contributor.author | Stevenson, J | |
dc.contributor.author | Barrow-McGee, R | |
dc.contributor.author | Yu, L | |
dc.contributor.author | Paul, A | |
dc.contributor.author | Mansfield, D | |
dc.contributor.author | Owen, J | |
dc.contributor.author | Woodman, N | |
dc.contributor.author | Natrajan, R | |
dc.contributor.author | Haider, S | |
dc.contributor.author | Gillett, C | |
dc.contributor.author | Tutt, A | |
dc.contributor.author | Pinder, SE | |
dc.contributor.author | Choudary, J | |
dc.contributor.author | Naidoo, K | |
dc.date.accessioned | 2021-03-31T10:16:06Z | |
dc.date.available | 2021-03-31T10:16:06Z | |
dc.date.issued | 2021-03-05 | |
dc.identifier.citation | NPJ breast cancer, 2021, 7 (1), pp. 24 - ? | |
dc.identifier.issn | 2374-4677 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/4474 | |
dc.identifier.eissn | 2374-4677 | |
dc.identifier.doi | 10.1038/s41523-021-00227-7 | |
dc.description.abstract | In breast cancer (BC), detecting low volumes of axillary lymph node (ALN) metastasis pre-operatively is difficult and novel biomarkers are needed. We recently showed that patient-derived ALNs can be sustained ex-vivo using normothermic perfusion. We now compare reactive (tumour-free; n = 5) and macrometastatic (containing tumour deposits >2 mm; n = 4) ALNs by combining whole section multiplex immunofluorescence with TMT-labelled LC-MS/MS of the circulating perfusate. Macrometastases contained significantly fewer B cells and T cells (CD4+/CD8+/regulatory) than reactive nodes (p = 0.02). Similarly, pathway analysis of the perfusate proteome (119/1453 proteins significantly differentially expressed) showed that immune function was diminished in macrometastases in favour of 'extracellular matrix degradation'; only 'neutrophil degranulation' was preserved. Qualitative comparison of the perfusate proteome to that of node-positive pancreatic and prostatic adenocarcinoma also highlighted 'neutrophil degranulation' as a contributing factor to nodal metastasis. Thus, metastasis-induced changes in the REPLICANT perfusate proteome are detectable, and could facilitate biomarker discovery. | |
dc.format | Electronic | |
dc.format.extent | 24 - ? | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | NATURE PORTFOLIO | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.title | Proteomics of REPLICANT perfusate detects changes in the metastatic lymph node microenvironment. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2021-01-20 | |
rioxxterms.version | VoR | |
rioxxterms.versionofrecord | 10.1038/s41523-021-00227-7 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2021-03-05 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | NPJ breast cancer | |
pubs.issue | 1 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research/Functional Genomics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Functional Genomics | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research/Functional Genomics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Functional Genomics | |
pubs.publication-status | Published | |
pubs.volume | 7 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Functional Genomics | |
icr.researchteam | Functional Genomics | |
dc.contributor.icrauthor | Natrajan, Rachael | |
dc.contributor.icrauthor | Haider, Syed | |
dc.contributor.icrauthor | Tutt, Andrew | |