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dc.contributor.authorCutts, EE
dc.contributor.authorTaylor, GC
dc.contributor.authorPardo, M
dc.contributor.authorYu, L
dc.contributor.authorWills, JC
dc.contributor.authorChoudhary, JS
dc.contributor.authorVannini, A
dc.contributor.authorWood, AJ
dc.date.accessioned2021-04-09T13:22:29Z
dc.date.available2021-04-09T13:22:29Z
dc.date.issued2021-01-13
dc.identifier.citationWellcome open research, 2021, 6 pp. 3 - ?
dc.identifier.issn2398-502X
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4509
dc.identifier.eissn2398-502X
dc.identifier.eissn2398-502Xen_US
dc.identifier.doi10.12688/wellcomeopenres.16482.1
dc.identifier.doi10.12688/wellcomeopenres.16482.1en_US
dc.description.abstractCondensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have assessed the specificity of a polyclonal antibody (Bethyl A302-276A) that is commonly used as a probe for NCAPH2, the kleisin subunit of condensin II, in mammalian cells. We find that, in addition to its intended target, this antibody cross-reacts with one or more components of the SWI/SNF family of chromatin remodelling complexes in an NCAPH2-independent manner. This cross-reactivity, with an abundant chromatin-associated factor, is likely to affect the interpretation of protein and chromatin immunoprecipitation experiments that make use of this antibody probe.
dc.formatElectronic-eCollection
dc.format.extent3 - ?
dc.languageeng
dc.language.isoeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleA commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component.
dc.typeJournal Article
dcterms.dateAccepted2021-01-13
rioxxterms.versionVoR
rioxxterms.versionofrecord10.12688/wellcomeopenres.16482.1
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2021-01-13
rioxxterms.licenseref.startdate2021-01-13en_US
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfWellcome open research
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Functional Proteomics Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology/Vannini Group
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Functional Proteomics Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology/Vannini Group
pubs.publication-statusPublished
pubs.volume6en_US
pubs.embargo.termsNot known
icr.researchteamFunctional Proteomics Group
icr.researchteamVannini Group
icr.researchteamFunctional Proteomics Groupen_US
icr.researchteamVannini Groupen_US
dc.contributor.icrauthorPardo Calvo, Maria Mercedesen
dc.contributor.icrauthorVannini, Alessandroen


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/