New Fully Automated Preparation of High Apparent Molar Activity 68Ga-FAPI-46 on a Trasis AiO Platform.
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Date
2022-01-20Author
Da Pieve, C
Costa Braga, M
Turton, DR
Valla, FA
Cakmak, P
Plate, K-H
Kramer-Marek, G
Type
Journal Article
Metadata
Show full item recordAbstract
A large number of applications for fibroblast activation protein inhibitors (FAPI)-based PET agents have been evaluated in conditions ranging from cancer to non-malignant diseases such as myocardial infarction. In particular, 68Ga-FAPI-46 was reported to have a high specificity and affinity for FAP-expressing cells, a fast and high accumulation in tumor lesions/injuries together with a fast body clearance when investigated in vivo. Due to the increasing interest in the use of the agent both preclinically and clinically, we developed an automated synthesis for the production of 68Ga-FAPI-46 on a Trasis AiO platform. The new synthetic procedure, which included the processing of the generator eluate using a strong cation exchange resin and a final purification step through an HLB followed by a QMA cartridge, yielded 68Ga-FAPI-46 with high radiochemical purity (>98%) and apparent molar activity (271.1 ± 105.6 MBq/nmol). Additionally, the in vitro and in vivo properties of the product were assessed on glioblastoma cells and mouse model. Although developed for the preparation of 68Ga-FAPI-46 for preclinical use, our method can be adapted for clinical production as a reliable alternative to the manual (i.e., cold kit) or modular systems preparations already described in the literature.
Collections
Subject
Tumor Cells, Cultured
Animals
Humans
Mice
Mice, Nude
Glioblastoma
Quinolines
Radiopharmaceuticals
Xenograft Model Antitumor Assays
Apoptosis
Cell Proliferation
Radiochemistry
Female
Positron Emission Tomography Computed Tomography
Research team
Preclinical Molecular Imaging
Language
eng
Date accepted
2022-01-17
License start date
2022-01-20
Citation
Molecules (Basel, Switzerland), 2022, 27 (3)
Publisher
MDPI