Papillary Thyroid Carcinoma: Molecular Distinction by MicroRNA Profiling.
Date
2022-02-23ICR Author
Author
Galuppini, F
Censi, S
Merante Boschin, I
Fassan, M
Sbaraglia, M
Valeri, N
Hahne, JC
Bertazza, L
Munari, G
Galasso, M
Cascione, L
Barollo, S
Rugge, M
Vianello, F
Dei Tos, AP
Mian, C
Pennelli, G
Type
Journal Article
Metadata
Show full item recordAbstract
Papillary thyroid carcinoma (PTC) is a miscellaneous disease with a variety of histological variants, each with its own mutational profile, and clinical and prognostic characteristics. Identification of microRNA (miRNA) expression profiles represents an important benchmark for understanding the molecular mechanisms underlying the biological behavior of these unique PTC subtypes in order that they be better characterized. We considered a series of 35 PTC samples with a histological diagnosis of either hobnail (17 cases) or classical variant (nine cases) and with a specific BRAF p.K601E mutation (nine cases). We determined the overall miRNA expression profile with NanoString technology, and both quantitative reverse transcription-PCR and in situ hybridization were used to confirm selected miRNAs. The miRNA signature was found to consistently differentiate specific histotypes and mutational profiles. In contrast to the BRAF p.K601E mutation and classic PTCs, three miRNAs (miR-21-5p, miR-146b-5p, and miR-205-5p) were substantially overexpressed in the hobnail variant. The current study found that different miRNA signature profiles were linked to unique histological variants and BRAF mutations in PTC. Further studies focusing on the downstream pathogenetic functions of mRNAs in thyroid neoplasms are warranted.
Collections
Subject
BRAF
histological variant
hobnail
miR-146
miR-205
miR-21
microRNA
papillary thyroid cancer
Carcinoma, Papillary
Humans
MicroRNAs
Proto-Oncogene Proteins B-raf
Thyroid Cancer, Papillary
Thyroid Neoplasms
Research team
GI Cancer Biol & Genomics
Language
eng
Date accepted
2022-01-26
License start date
2022-02-23
Citation
Frontiers in Endocrinology, 2022, 13 pp. 834075 -
Publisher
FRONTIERS MEDIA SA