dc.contributor.author | Neyen, C | |
dc.contributor.author | Runchel, C | |
dc.contributor.author | Schüpfer, F | |
dc.contributor.author | Meier, P | |
dc.contributor.author | Lemaitre, B | |
dc.date.accessioned | 2017-03-24T15:11:17Z | |
dc.date.issued | 2016-08-22 | |
dc.identifier.citation | Nature immunology, 2016, 17 (10), pp. 1150 - 1158 | |
dc.identifier.issn | 1529-2908 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/525 | |
dc.identifier.eissn | 1529-2916 | |
dc.identifier.doi | 10.1038/ni.3536 | |
dc.description.abstract | The innate immune system needs to distinguish between harmful and innocuous stimuli to adapt its activation to the level of threat. How Drosophila mounts differential immune responses to dead and live Gram-negative bacteria using the single peptidoglycan receptor PGRP-LC is unknown. Here we describe rPGRP-LC, an alternative splice variant of PGRP-LC that selectively dampens immune response activation in response to dead bacteria. rPGRP-LC-deficient flies cannot resolve immune activation after Gram-negative infection and die prematurely. The alternative exon in the encoding gene, here called rPGRP-LC, encodes an adaptor module that targets rPGRP-LC to membrane microdomains and interacts with the negative regulator Pirk and the ubiquitin ligase DIAP2. We find that rPGRP-LC-mediated resolution of an efficient immune response requires degradation of activating and regulatory receptors via endosomal ESCRT sorting. We propose that rPGRP-LC selectively responds to peptidoglycans from dead bacteria to tailor the immune response to the level of threat. | |
dc.format | Print-Electronic | |
dc.format.extent | 1150 - 1158 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | NATURE PUBLISHING GROUP | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Cell Line | |
dc.subject | Membrane Microdomains | |
dc.subject | Endosomes | |
dc.subject | Animals | |
dc.subject | Animals, Genetically Modified | |
dc.subject | Pectobacterium carotovorum | |
dc.subject | Gram-Negative Bacterial Infections | |
dc.subject | Protein Sorting Signals | |
dc.subject | Carrier Proteins | |
dc.subject | Drosophila Proteins | |
dc.subject | Immunity | |
dc.subject | Protein Binding | |
dc.subject | Structure-Activity Relationship | |
dc.subject | Exons | |
dc.subject | Inhibitor of Apoptosis Proteins | |
dc.subject | Gene Knockout Techniques | |
dc.subject | Immunomodulation | |
dc.subject | Endosomal Sorting Complexes Required for Transport | |
dc.subject | Proteolysis | |
dc.subject | RNA Isoforms | |
dc.title | The regulatory isoform rPGRP-LC induces immune resolution via endosomal degradation of receptors. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2016-07-18 | |
rioxxterms.versionofrecord | 10.1038/ni.3536 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2016-10 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Nature immunology | |
pubs.issue | 10 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research/Cell Death and Immunity | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research/Cell Death and Immunity | |
pubs.publication-status | Published | |
pubs.volume | 17 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Cell Death and Immunity | |
dc.contributor.icrauthor | Meier, Pascal | |