Super-resolution microscopy: a brief history and new avenues.
View/ Open
Date
2022-04-04ICR Author
Author
Prakash, K
Diederich, B
Heintzmann, R
Schermelleh, L
Type
Journal Article
Metadata
Show full item recordAbstract
Super-resolution microscopy (SRM) is a fast-developing field that encompasses fluorescence imaging techniques with the capability to resolve objects below the classical diffraction limit of optical resolution. Acknowledged with the Nobel prize in 2014, numerous SRM methods have meanwhile evolved and are being widely applied in biomedical research, all with specific strengths and shortcomings. While some techniques are capable of nanometre-scale molecular resolution, others are geared towards volumetric three-dimensional multi-colour or fast live-cell imaging. In this editorial review, we pick on the latest trends in the field. We start with a brief historical overview of both conceptual and commercial developments. Next, we highlight important parameters for imaging successfully with a particular super-resolution modality. Finally, we discuss the importance of reproducibility and quality control and the significance of open-source tools in microscopy. This article is part of the Theo Murphy meeting issue 'Super-resolution structured illumination microscopy (part 2)'.
Collections
Research team
Integrated Pathology
Language
eng
Date accepted
2022-02-14
License start date
2022-04-04
Citation
Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 2022, 380 (2220),
Publisher
ROYAL SOC