dc.contributor.author | van Maldegem, F | |
dc.contributor.author | Valand, K | |
dc.contributor.author | Cole, M | |
dc.contributor.author | Patel, H | |
dc.contributor.author | Angelova, M | |
dc.contributor.author | Rana, S | |
dc.contributor.author | Colliver, E | |
dc.contributor.author | Enfield, K | |
dc.contributor.author | Bah, N | |
dc.contributor.author | Kelly, G | |
dc.contributor.author | Tsang, VSK | |
dc.contributor.author | Mugarza, E | |
dc.contributor.author | Moore, C | |
dc.contributor.author | Hobson, P | |
dc.contributor.author | Levi, D | |
dc.contributor.author | Molina-Arcas, M | |
dc.contributor.author | Swanton, C | |
dc.contributor.author | Downward, J | |
dc.coverage.spatial | England | |
dc.date.accessioned | 2022-09-02T08:12:08Z | |
dc.date.available | 2022-09-02T08:12:08Z | |
dc.date.issued | 2021-10-08 | |
dc.identifier | ARTN 5906 | |
dc.identifier | 10.1038/s41467-021-26214-x | |
dc.identifier.citation | Nature Communications, 2021, 12 (1), pp. 5906 - | en_US |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/5371 | |
dc.identifier.eissn | 2041-1723 | |
dc.identifier.eissn | 2041-1723 | |
dc.identifier.doi | 10.1038/s41467-021-26214-x | |
dc.description.abstract | Mouse models are critical in pre-clinical studies of cancer therapy, allowing dissection of mechanisms through chemical and genetic manipulations that are not feasible in the clinical setting. In studies of the tumour microenvironment (TME), multiplexed imaging methods can provide a rich source of information. However, the application of such technologies in mouse tissues is still in its infancy. Here we present a workflow for studying the TME using imaging mass cytometry with a panel of 27 antibodies on frozen mouse tissues. We optimise and validate image segmentation strategies and automate the process in a Nextflow-based pipeline (imcyto) that is scalable and portable, allowing for parallelised segmentation of large multi-image datasets. With these methods we interrogate the remodelling of the TME induced by a KRAS G12C inhibitor in an immune competent mouse orthotopic lung cancer model, highlighting the infiltration and activation of antigen presenting cells and effector cells. | |
dc.format | Electronic | |
dc.format.extent | 5906 - | |
dc.language | eng | |
dc.language.iso | eng | en_US |
dc.publisher | NATURE PORTFOLIO | en_US |
dc.relation.ispartof | Nature Communications | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Animals | |
dc.subject | Antibodies | |
dc.subject | Antineoplastic Agents | |
dc.subject | Carcinoma, Non-Small-Cell Lung | |
dc.subject | Disease Models, Animal | |
dc.subject | Image Cytometry | |
dc.subject | Lung Neoplasms | |
dc.subject | Macrophages | |
dc.subject | Mice | |
dc.subject | Mice, Inbred C57BL | |
dc.subject | Oncogenes | |
dc.subject | T-Lymphocytes | |
dc.subject | Tumor Microenvironment | |
dc.title | Characterisation of tumour microenvironment remodelling following oncogene inhibition in preclinical studies with imaging mass cytometry. | en_US |
dc.type | Journal Article | |
dcterms.dateAccepted | 2021-09-20 | |
dc.date.updated | 2022-09-02T08:11:30Z | |
rioxxterms.version | VoR | en_US |
rioxxterms.versionofrecord | 10.1038/s41467-021-26214-x | en_US |
rioxxterms.licenseref.startdate | 2021-10-08 | |
rioxxterms.type | Journal Article/Review | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/34625563 | |
pubs.issue | 1 | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Lung Cancer Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Lung Cancer Group | |
pubs.publication-status | Published online | |
pubs.publisher-url | http://dx.doi.org/10.1038/s41467-021-26214-x | |
pubs.volume | 12 | |
icr.researchteam | Lung Cancer Group | en_US |
dc.contributor.icrauthor | Downward, Julian David Harry | |
icr.provenance | Deposited by Mr Arek Surman on 2022-09-02. Deposit type is initial. No. of files: 1. Files: Characterisation of tumour microenvironment remodelling following oncogene inhibition in preclinical studies with imaging ma.pdf | |