RNA sequencing-based single sample predictors of molecular subtype and risk of recurrence for clinical assessment of early-stage breast cancer.
Date
2022-08-16ICR Author
Author
Staaf, J
Häkkinen, J
Hegardt, C
Saal, LH
Kimbung, S
Hedenfalk, I
Lien, T
Sørlie, T
Naume, B
Russnes, H
Marcone, R
Ayyanan, A
Brisken, C
Malterling, RR
Asking, B
Olofsson, H
Lindman, H
Bendahl, P-O
Ehinger, A
Larsson, C
Loman, N
Rydén, L
Malmberg, M
Borg, Å
Vallon-Christersson, J
Type
Journal Article
Metadata
Show full item recordAbstract
Multigene assays for molecular subtypes and biomarkers can aid management of early invasive breast cancer. Using RNA-sequencing we aimed to develop single-sample predictor (SSP) models for clinical markers, subtypes, and risk of recurrence (ROR). A cohort of 7743 patients was divided into training and test set. We trained SSPs for subtypes and ROR assigned by nearest-centroid (NC) methods and SSPs for biomarkers from histopathology. Classifications were compared with Prosigna in two external cohorts (ABiM, n = 100 and OSLO2-EMIT0, n = 103). Prognostic value was assessed using distant recurrence-free interval. Agreement between SSP and NC for PAM50 (five subtypes) was high (85%, Kappa = 0.78) for Subtype (four subtypes) very high (90%, Kappa = 0.84) and for ROR risk category high (84%, Kappa = 0.75, weighted Kappa = 0.90). Prognostic value was assessed as equivalent and clinically relevant. Agreement with histopathology was very high or high for receptor status, while moderate for Ki67 status and poor for Nottingham histological grade. SSP and Prosigna concordance was high for subtype (OSLO-EMIT0 83%, Kappa = 0.73 and ABiM 80%, Kappa = 0.72) and moderate and high for ROR risk category (68 and 84%, Kappa = 0.50 and 0.70, weighted Kappa = 0.70 and 0.78). Pooled concordance for emulated treatment recommendation dichotomized for chemotherapy was high (85%, Kappa = 0.66). Retrospective evaluation suggested that SSP application could change chemotherapy recommendations for up to 17% of postmenopausal ER+/HER2-/N0 patients with balanced escalation and de-escalation. Results suggest that NC and SSP models are interchangeable on a group-level and nearly so on a patient level and that SSP models can be derived to closely match clinical tests.
Collections
Subject
Science & Technology
Life Sciences & Biomedicine
Oncology
HISTOLOGIC GRADE
PAM50 RISK
ALIGNMENT
THERAPY
ABCSG-8
WOMEN
HISAT
SCORE
Research team
Endocrine control mechans
Language
eng
Date accepted
2022-07-20
License start date
2022-08-16
Citation
npj Breast Cancer, 2022, 8 (1), pp. 94 -
Publisher
NATURE PORTFOLIO