Phenotypic plasticity and genetic control in colorectal cancer evolution.
Date
2022-11-24ICR Author
Author
Househam, J
Heide, T
Cresswell, GD
Spiteri, I
Kimberley, C
Zapata, L
Lynn, C
James, C
Mossner, M
Fernandez-Mateos, J
Vinceti, A
Baker, A-M
Gabbutt, C
Berner, A
Schmidt, M
Chen, B
Lakatos, E
Gunasri, V
Nichol, D
Costa, H
Mitchinson, M
Ramazzotti, D
Werner, B
Iorio, F
Jansen, M
Caravagna, G
Barnes, CP
Shibata, D
Bridgewater, J
Rodriguez-Justo, M
Magnani, L
Sottoriva, A
Graham, TA
Type
Journal Article
Metadata
Show full item recordAbstract
Genetic and epigenetic variation, together with transcriptional plasticity, contribute to intratumour heterogeneity1. The interplay of these biological processes and their respective contributions to tumour evolution remain unknown. Here we show that intratumour genetic ancestry only infrequently affects gene expression traits and subclonal evolution in colorectal cancer (CRC). Using spatially resolved paired whole-genome and transcriptome sequencing, we find that the majority of intratumour variation in gene expression is not strongly heritable but rather 'plastic'. Somatic expression quantitative trait loci analysis identified a number of putative genetic controls of expression by cis-acting coding and non-coding mutations, the majority of which were clonal within a tumour, alongside frequent structural alterations. Consistently, computational inference on the spatial patterning of tumour phylogenies finds that a considerable proportion of CRCs did not show evidence of subclonal selection, with only a subset of putative genetic drivers associated with subclone expansions. Spatial intermixing of clones is common, with some tumours growing exponentially and others only at the periphery. Together, our data suggest that most genetic intratumour variation in CRC has no major phenotypic consequence and that transcriptional plasticity is, instead, widespread within a tumour.
Collections
Research team
Genomics & evolut dynam
Evol Genomics & Modelling
Language
eng
Date accepted
2022-09-01
License start date
2022-10-26
Citation
Nature, 2022,
Publisher
NATURE PORTFOLIO