dc.contributor.author | Koliopoulos, MG | |
dc.contributor.author | Muhammad, R | |
dc.contributor.author | Roumeliotis, TI | |
dc.contributor.author | Beuron, F | |
dc.contributor.author | Choudhary, JS | |
dc.contributor.author | Alfieri, C | |
dc.coverage.spatial | England | |
dc.date.accessioned | 2022-11-16T15:26:40Z | |
dc.date.available | 2022-11-16T15:26:40Z | |
dc.date.issued | 2022-08-29 | |
dc.identifier | ARTN 5075 | |
dc.identifier | 10.1038/s41467-022-32798-9 | |
dc.identifier.citation | Nature Communications, 2022, 13 (1), pp. 5075 - | en_US |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/5564 | |
dc.identifier.eissn | 2041-1723 | |
dc.identifier.eissn | 2041-1723 | |
dc.identifier.doi | 10.1038/s41467-022-32798-9 | |
dc.description.abstract | Genes encoding the core cell cycle machinery are transcriptionally regulated by the MuvB family of protein complexes in a cell cycle-specific manner. Complexes of MuvB with the transcription factors B-MYB and FOXM1 activate mitotic genes during cell proliferation. The mechanisms of transcriptional regulation by these complexes are still poorly characterised. Here, we combine biochemical analysis and in vitro reconstitution, with structural analysis by cryo-electron microscopy and cross-linking mass spectrometry, to functionally examine these complexes. We find that the MuvB:B-MYB complex binds and remodels nucleosomes, thereby exposing nucleosomal DNA. This remodelling activity is supported by B-MYB which directly binds the remodelled DNA. Given the remodelling activity on the nucleosome, we propose that the MuvB:B-MYB complex functions as a pioneer transcription factor complex. In this work, we rationalise prior biochemical and cellular studies and provide a molecular framework of interactions on a protein complex that is key for cell cycle regulation. | |
dc.format | Electronic | |
dc.format.extent | 5075 - | |
dc.language | eng | |
dc.language.iso | eng | en_US |
dc.publisher | NATURE PORTFOLIO | en_US |
dc.relation.ispartof | Nature Communications | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Cell Cycle | |
dc.subject | Cryoelectron Microscopy | |
dc.subject | DNA | |
dc.subject | Gene Expression Regulation | |
dc.subject | Nucleosomes | |
dc.subject | Transcription Factors | |
dc.title | Structure of a nucleosome-bound MuvB transcription factor complex reveals DNA remodelling. | en_US |
dc.type | Journal Article | |
dcterms.dateAccepted | 2022-08-15 | |
dc.date.updated | 2022-11-16T15:26:02Z | |
rioxxterms.version | VoR | en_US |
rioxxterms.versionofrecord | 10.1038/s41467-022-32798-9 | en_US |
rioxxterms.licenseref.startdate | 2022-08-29 | |
rioxxterms.type | Journal Article/Review | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/36038598 | |
pubs.issue | 1 | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Structural Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Functional Proteomics Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Structural Biology/Molecular mechanisms of cell cycle regulation | |
pubs.organisational-group | /ICR/ImmNet | |
pubs.publication-status | Published online | |
pubs.publisher-url | http://dx.doi.org/10.1038/s41467-022-32798-9 | |
pubs.volume | 13 | |
icr.researchteam | Functional Proteomics | en_US |
icr.researchteam | Electron Microscopy Fac | en_US |
icr.researchteam | Prote & Metabolomics Fac | en_US |
icr.researchteam | Mol mech cell cycle reg | en_US |
dc.contributor.icrauthor | Roumeliotis, Theodoros | |
dc.contributor.icrauthor | Beuron, Fabienne | |
dc.contributor.icrauthor | Choudhary, Jyoti | |
dc.contributor.icrauthor | Alfieri, Claudio | |
icr.provenance | Deposited by Mr Arek Surman on 2022-11-16. Deposit type is initial. No. of files: 1. Files: Structure of a nucleosome-bound MuvB transcription factor complex reveals DNA remodelling.pdf | |