dc.contributor.author | Quicke, P | |
dc.contributor.author | Sun, Y | |
dc.contributor.author | Arias-Garcia, M | |
dc.contributor.author | Beykou, M | |
dc.contributor.author | Acker, CD | |
dc.contributor.author | Djamgoz, MBA | |
dc.contributor.author | Bakal, C | |
dc.contributor.author | Foust, AJ | |
dc.coverage.spatial | England | |
dc.date.accessioned | 2023-01-31T10:00:24Z | |
dc.date.available | 2023-01-31T10:00:24Z | |
dc.date.issued | 2022-11-11 | |
dc.identifier | ARTN 1178 | |
dc.identifier | 10.1038/s42003-022-04077-2 | |
dc.identifier.citation | Communications Biology, 2022, 5 (1), pp. 1178 - | en_US |
dc.identifier.issn | 2399-3642 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/5672 | |
dc.identifier.eissn | 2399-3642 | |
dc.identifier.eissn | 2399-3642 | |
dc.identifier.doi | 10.1038/s42003-022-04077-2 | |
dc.description.abstract | Cancer cells feature a resting membrane potential (Vm) that is depolarized compared to normal cells, and express active ionic conductances, which factor directly in their pathophysiological behavior. Despite similarities to 'excitable' tissues, relatively little is known about cancer cell Vm dynamics. Here high-throughput, cellular-resolution Vm imaging reveals that Vm fluctuates dynamically in several breast cancer cell lines compared to non-cancerous MCF-10A cells. We characterize Vm fluctuations of hundreds of human triple-negative breast cancer MDA-MB-231 cells. By quantifying their Dynamic Electrical Signatures (DESs) through an unsupervised machine-learning protocol, we identify four classes ranging from "noisy" to "blinking/waving". The Vm of MDA-MB-231 cells exhibits spontaneous, transient hyperpolarizations inhibited by the voltage-gated sodium channel blocker tetrodotoxin, and by calcium-activated potassium channel inhibitors apamin and iberiotoxin. The Vm of MCF-10A cells is comparatively static, but fluctuations increase following treatment with transforming growth factor-β1, a canonical inducer of the epithelial-to-mesenchymal transition. These data suggest that the ability to generate Vm fluctuations may be a property of hybrid epithelial-mesenchymal cells or those originated from luminal progenitors. | |
dc.format | Electronic | |
dc.format.extent | 1178 - | |
dc.language | eng | |
dc.language.iso | eng | en_US |
dc.publisher | NATURE PORTFOLIO | en_US |
dc.relation.ispartof | Communications Biology | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | Humans | |
dc.subject | Cell Line, Tumor | |
dc.subject | Epithelial-Mesenchymal Transition | |
dc.subject | Triple Negative Breast Neoplasms | |
dc.subject | MCF-7 Cells | |
dc.subject | Membrane Potentials | |
dc.title | Voltage imaging reveals the dynamic electrical signatures of human breast cancer cells. | en_US |
dc.type | Journal Article | |
dcterms.dateAccepted | 2022-10-05 | |
dc.date.updated | 2023-01-31T09:59:25Z | |
rioxxterms.version | VoR | en_US |
rioxxterms.versionofrecord | 10.1038/s42003-022-04077-2 | en_US |
rioxxterms.licenseref.startdate | 2022-11-11 | |
rioxxterms.type | Journal Article/Review | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/36369329 | |
pubs.issue | 1 | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Dynamical Cell Systems | |
pubs.publication-status | Published online | |
pubs.publisher-url | http://dx.doi.org/10.1038/s42003-022-04077-2 | |
pubs.volume | 5 | |
icr.researchteam | Dynamical Cell Systems | en_US |
dc.contributor.icrauthor | Bakal, Christopher | |
icr.provenance | Deposited by Mr Arek Surman on 2023-01-31. Deposit type is initial. No. of files: 1. Files: Voltage imaging reveals the dynamic electrical signatures of human breast cancer cells.pdf | |