dc.contributor.author | Russell, AJC | |
dc.contributor.author | Sanderson, T | |
dc.contributor.author | Bushell, E | |
dc.contributor.author | Talman, AM | |
dc.contributor.author | Anar, B | |
dc.contributor.author | Girling, G | |
dc.contributor.author | Hunziker, M | |
dc.contributor.author | Kent, RS | |
dc.contributor.author | Martin, JS | |
dc.contributor.author | Metcalf, T | |
dc.contributor.author | Montandon, R | |
dc.contributor.author | Pandey, V | |
dc.contributor.author | Pardo, M | |
dc.contributor.author | Roberts, AB | |
dc.contributor.author | Sayers, C | |
dc.contributor.author | Schwach, F | |
dc.contributor.author | Choudhary, JS | |
dc.contributor.author | Rayner, JC | |
dc.contributor.author | Voet, T | |
dc.contributor.author | Modrzynska, KK | |
dc.contributor.author | Waters, AP | |
dc.contributor.author | Lawniczak, MKN | |
dc.contributor.author | Billker, O | |
dc.date.accessioned | 2023-05-18T14:58:57Z | |
dc.date.available | 2023-05-18T14:58:57Z | |
dc.date.issued | 2023-02-08 | |
dc.identifier.citation | Cell Host and Microbe, 2023, 31 (2), pp. 305 - + | |
dc.identifier.issn | 1931-3128 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/5788 | |
dc.identifier.eissn | 1934-6069 | |
dc.identifier.eissn | 1934-6069 | |
dc.identifier.doi | 10.1016/j.chom.2022.12.011 | |
dc.description.abstract | Malaria transmission to mosquitoes requires a developmental switch in asexually dividing blood-stage parasites to sexual reproduction. In Plasmodium berghei, the transcription factor AP2-G is required and sufficient for this switch, but how a particular sex is determined in a haploid parasite remains unknown. Using a global screen of barcoded mutants, we here identify genes essential for the formation of either male or female sexual forms and validate their importance for transmission. High-resolution single-cell transcriptomics of ten mutant parasites portrays the developmental bifurcation and reveals a regulatory cascade of putative gene functions in the determination and subsequent differentiation of each sex. A male-determining gene with a LOTUS/OST-HTH domain as well as the protein interactors of a female-determining zinc-finger protein indicate that germ-granule-like ribonucleoprotein complexes complement transcriptional processes in the regulation of both male and female development of a malaria parasite. | |
dc.format.extent | 305 - + | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | CELL PRESS | |
dc.relation.ispartof | Cell Host and Microbe | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Microbiology | |
dc.subject | Parasitology | |
dc.subject | Virology | |
dc.subject | PLASMODIUM | |
dc.subject | COMMITMENT | |
dc.subject | REPRESSION | |
dc.subject | PROTEINS | |
dc.subject | DATABASE | |
dc.subject | REVEALS | |
dc.subject | HISAT | |
dc.subject | LOTUS | |
dc.subject | RATIO | |
dc.title | Regulators of male and female sexual development are critical for the transmission of a malaria parasite. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2022-12-12 | |
dc.date.updated | 2023-05-16T08:14:13Z | |
rioxxterms.version | VoR | |
rioxxterms.versionofrecord | 10.1016/j.chom.2022.12.011 | |
rioxxterms.licenseref.startdate | 2023-02-08 | |
rioxxterms.type | Journal Article/Review | |
pubs.issue | 2 | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Functional Proteomics Group | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1016/j.chom.2022.12.011 | |
pubs.volume | 31 | |
icr.researchteam | Functional Proteomics | |
dc.contributor.icrauthor | Pardo Calvo, Maria Mercedes | |
dc.contributor.icrauthor | Choudhary, Jyoti | |
icr.provenance | Deposited by Dr Mercedes Pardo Calvo on 2023-05-16. Deposit type is initial. No. of files: 1. Files: Russell2023_malariasexgene.pdf | |