dc.contributor.author | Dent, LG | |
dc.contributor.author | Curry, N | |
dc.contributor.author | Sparks, H | |
dc.contributor.author | Bousgouni, V | |
dc.contributor.author | Maioli, V | |
dc.contributor.author | Kumar, S | |
dc.contributor.author | Munro, I | |
dc.contributor.author | Butera, F | |
dc.contributor.author | Jones, I | |
dc.contributor.author | Arias-Garcia, M | |
dc.contributor.author | Rowe-Brown, L | |
dc.contributor.author | Dunsby, C | |
dc.contributor.author | Bakal, C | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2024-07-03T12:31:10Z | |
dc.date.available | 2024-07-03T12:31:10Z | |
dc.date.issued | 2024-05-28 | |
dc.identifier | ARTN 114016 | |
dc.identifier | S2211-1247(24)00344-9 | |
dc.identifier.citation | Cell Reports, 2024, 43 (5), pp. 114016 - | en_US |
dc.identifier.issn | 2211-1247 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/6278 | |
dc.identifier.eissn | 2211-1247 | |
dc.identifier.eissn | 2211-1247 | |
dc.identifier.doi | 10.1016/j.celrep.2024.114016 | |
dc.identifier.doi | 10.1016/j.celrep.2024.114016 | |
dc.description.abstract | How cancer cells determine their shape in response to three-dimensional (3D) geometric and mechanical cues is unclear. We develop an approach to quantify the 3D cell shape of over 60,000 melanoma cells in collagen hydrogels using high-throughput stage-scanning oblique plane microscopy (ssOPM). We identify stereotypic and environmentally dependent changes in shape and protrusivity depending on whether a cell is proximal to a flat and rigid surface or is embedded in a soft environment. Environmental sensitivity metrics calculated for small molecules and gene knockdowns identify interactions between the environment and cellular factors that are important for morphogenesis. We show that the Rho guanine nucleotide exchange factor (RhoGEF) TIAM2 contributes to shape determination in environmentally independent ways but that non-muscle myosin II, microtubules, and the RhoGEF FARP1 regulate shape in ways dependent on the microenvironment. Thus, changes in cancer cell shape in response to 3D geometric and mechanical cues are modulated in both an environmentally dependent and independent fashion. | |
dc.format | Print-Electronic | |
dc.format.extent | 114016 - | |
dc.language | eng | |
dc.language.iso | eng | en_US |
dc.publisher | CELL PRESS | en_US |
dc.relation.ispartof | Cell Reports | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.subject | CP: Cell biology | |
dc.subject | FARP1 | |
dc.subject | OPM | |
dc.subject | RhoGEF | |
dc.subject | TIAM2 | |
dc.subject | cell protrusions | |
dc.subject | collagen | |
dc.subject | imaging 3D morphology | |
dc.subject | melanoma | |
dc.subject | microenvironment | |
dc.subject | oblique plane microscopy | |
dc.subject | Humans | |
dc.subject | Guanine Nucleotide Exchange Factors | |
dc.subject | Cell Shape | |
dc.subject | Cell Line, Tumor | |
dc.subject | Microtubules | |
dc.subject | Myosin Type II | |
dc.subject | Rho Guanine Nucleotide Exchange Factors | |
dc.subject | Melanoma | |
dc.title | Environmentally dependent and independent control of 3D cell shape. | en_US |
dc.type | Journal Article | |
dcterms.dateAccepted | 2024-03-14 | |
dc.date.updated | 2024-07-03T12:30:29Z | |
rioxxterms.version | VoR | en_US |
rioxxterms.versionofrecord | 10.1016/j.celrep.2024.114016 | en_US |
rioxxterms.licenseref.startdate | 2024-05-28 | |
rioxxterms.type | Journal Article/Review | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/38636520 | |
pubs.issue | 5 | |
pubs.organisational-group | ICR | |
pubs.organisational-group | ICR/Primary Group | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions/Cancer Biology/Dynamical Cell Systems | |
pubs.organisational-group | ICR/Students | |
pubs.organisational-group | ICR/Students/PhD and MPhil | |
pubs.organisational-group | ICR/Students/PhD and MPhil/17/18 Starting Cohort | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1016/j.celrep.2024.114016 | |
pubs.volume | 43 | |
icr.researchteam | Dynamical Cell Systems | en_US |
dc.contributor.icrauthor | Bousgouni, Paraskevi | |
dc.contributor.icrauthor | Bakal, Christopher | |
icr.provenance | Deposited by Mr Arek Surman on 2024-07-03. Deposit type is initial. No. of files: 1. Files: 1-s2.0-S2211124724003449-main.pdf | |