dc.contributor.author | Boumelha, J | |
dc.contributor.author | de Castro, A | |
dc.contributor.author | Bah, N | |
dc.contributor.author | Cha, H | |
dc.contributor.author | de Carné Trécesson, S | |
dc.contributor.author | Rana, S | |
dc.contributor.author | Tomaschko, M | |
dc.contributor.author | Anastasiou, P | |
dc.contributor.author | Mugarza, E | |
dc.contributor.author | Moore, C | |
dc.contributor.author | Goldstone, R | |
dc.contributor.author | East, P | |
dc.contributor.author | Litchfield, K | |
dc.contributor.author | Lee, S-H | |
dc.contributor.author | Molina-Arcas, M | |
dc.contributor.author | Downward, J | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2024-07-04T13:50:28Z | |
dc.date.available | 2024-07-04T13:50:28Z | |
dc.date.issued | 2024-04-18 | |
dc.identifier | 743103 | |
dc.identifier.citation | Cancer Research, 2024, | en_US |
dc.identifier.issn | 0008-5472 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/6298 | |
dc.identifier.eissn | 1538-7445 | |
dc.identifier.eissn | 1538-7445 | |
dc.identifier.doi | 10.1158/0008-5472.CAN-23-2627 | |
dc.identifier.doi | 10.1158/0008-5472.CAN-23-2627 | |
dc.description.abstract | Oncogenic KRAS impairs anti-tumor immune responses. As effective strategies to combine KRAS inhibitors and immunotherapies have so far proven elusive, a better understanding of how oncogenic KRAS drives immune evasion is needed to identify approaches that could sensitize KRAS-mutant lung cancer to immunotherapy. In vivo CRISPR-Cas9 screening in an immunogenic murine lung cancer model identified mechanisms by which oncogenic KRAS promotes immune evasion, most notably via upregulation of immunosuppressive cyclooxygenase-2 (COX-2) in cancer cells. Oncogenic KRAS potently induced COX-2 in both mouse and human lung cancer, which was suppressed using KRAS inhibitors. COX-2 acted via prostaglandin E2 (PGE2) to promote resistance to immune checkpoint blockade (ICB) in lung adenocarcinoma. Targeting COX-2/PGE2 remodeled the tumor microenvironment by inducing pro-inflammatory polarization of myeloid cells and influx of activated cytotoxic CD8+ T cells, which increased the efficacy of ICB. Restoration of COX-2 expression contributed to tumor relapse after prolonged KRAS inhibition. These results provide the rationale for testing COX-2/PGE2 pathway inhibitors in combination with KRASG12C inhibition or ICB in patients with KRAS-mutant lung cancer. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.language.iso | eng | en_US |
dc.publisher | American Association for Cancer Research (AACR) | en_US |
dc.relation.ispartof | Cancer Research | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.title | CRISPR-Cas9 screening identifies KRAS-induced COX-2 as a driver of immunotherapy resistance in lung cancer. | en_US |
dc.type | Journal Article | |
dcterms.dateAccepted | 2024-04-08 | |
dc.date.updated | 2024-07-04T13:49:48Z | |
rioxxterms.version | AM | en_US |
rioxxterms.versionofrecord | 10.1158/0008-5472.CAN-23-2627 | en_US |
rioxxterms.licenseref.startdate | 2024-04-18 | |
rioxxterms.type | Journal Article/Review | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/38635884 | |
pubs.organisational-group | ICR | |
pubs.organisational-group | ICR/Primary Group | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions/Cancer Biology/Lung Cancer Group | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions/Closed research teams | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions/Closed research teams/Lung Cancer Group | |
pubs.publication-status | Published online | |
pubs.publisher-url | http://dx.doi.org/10.1158/0008-5472.can-23-2627 | |
icr.researchteam | Lung Cancer Group | en_US |
dc.contributor.icrauthor | Downward, Julian David Harry | |
icr.provenance | Deposited by Mr Arek Surman on 2024-07-04. Deposit type is initial. No. of files: 1. Files: can-23-2627.pdf | |