dc.contributor.author | Welby, E | |
dc.contributor.author | Lakowski, J | |
dc.contributor.author | Di Foggia, V | |
dc.contributor.author | Budinger, D | |
dc.contributor.author | Gonzalez-Cordero, A | |
dc.contributor.author | Lun, ATL | |
dc.contributor.author | Epstein, M | |
dc.contributor.author | Patel, A | |
dc.contributor.author | Cuevas, E | |
dc.contributor.author | Kruczek, K | |
dc.contributor.author | Naeem, A | |
dc.contributor.author | Minneci, F | |
dc.contributor.author | Hubank, M | |
dc.contributor.author | Jones, DT | |
dc.contributor.author | Marioni, JC | |
dc.contributor.author | Ali, RR | |
dc.contributor.author | Sowden, JC | |
dc.date.accessioned | 2017-12-19T10:45:17Z | |
dc.date.issued | 2017-12 | |
dc.identifier.citation | Stem cell reports, 2017, 9 (6), pp. 1898 - 1915 | |
dc.identifier.issn | 2213-6711 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/975 | |
dc.identifier.eissn | 2213-6711 | |
dc.identifier.doi | 10.1016/j.stemcr.2017.10.018 | |
dc.description.abstract | Loss of cone photoreceptors, crucial for daylight vision, has the greatest impact on sight in retinal degeneration. Transplantation of stem cell-derived L/M-opsin cones, which form 90% of the human cone population, could provide a feasible therapy to restore vision. However, transcriptomic similarities between fetal and stem cell-derived cones remain to be defined, in addition to development of cone cell purification strategies. Here, we report an analysis of the human L/M-opsin cone photoreceptor transcriptome using an AAV2/9.pR2.1:GFP reporter. This led to the identification of a cone-enriched gene signature, which we used to demonstrate similar gene expression between fetal and stem cell-derived cones. We then defined a cluster of differentiation marker combination that, when used for cell sorting, significantly enriches for cone photoreceptors from the fetal retina and stem cell-derived retinal organoids, respectively. These data may facilitate more efficient isolation of human stem cell-derived cones for use in clinical transplantation studies. | |
dc.format | Print-Electronic | |
dc.format.extent | 1898 - 1915 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Retina | |
dc.subject | Fetus | |
dc.subject | Humans | |
dc.subject | Retinal Degeneration | |
dc.subject | Gene Expression Profiling | |
dc.subject | Cell Differentiation | |
dc.subject | Gene Expression Regulation, Developmental | |
dc.subject | Rod Opsins | |
dc.subject | Retinal Cone Photoreceptor Cells | |
dc.subject | Induced Pluripotent Stem Cells | |
dc.subject | Transcriptome | |
dc.title | Isolation and Comparative Transcriptome Analysis of Human Fetal and iPSC-Derived Cone Photoreceptor Cells. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2017-10-15 | |
rioxxterms.versionofrecord | 10.1016/j.stemcr.2017.10.018 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2017-12 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Stem cell reports | |
pubs.issue | 6 | |
pubs.notes | No embargo | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Translational Genomics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Translational Genomics/Translational Genomics (hon.) | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Translational Genomics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Translational Genomics/Translational Genomics (hon.) | |
pubs.publication-status | Published | |
pubs.volume | 9 | |
pubs.embargo.terms | No embargo | |
icr.researchteam | Translational Genomics | en_US |
dc.contributor.icrauthor | Hubank, Michael | en |