Isolation and Comparative Transcriptome Analysis of Human Fetal and iPSC-Derived Cone Photoreceptor Cells.
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Date
2017-12ICR Author
Author
Welby, E
Lakowski, J
Di Foggia, V
Budinger, D
Gonzalez-Cordero, A
Lun, ATL
Epstein, M
Patel, A
Cuevas, E
Kruczek, K
Naeem, A
Minneci, F
Hubank, M
Jones, DT
Marioni, JC
Ali, RR
Sowden, JC
Type
Journal Article
Metadata
Show full item recordAbstract
Loss of cone photoreceptors, crucial for daylight vision, has the greatest impact on sight in retinal degeneration. Transplantation of stem cell-derived L/M-opsin cones, which form 90% of the human cone population, could provide a feasible therapy to restore vision. However, transcriptomic similarities between fetal and stem cell-derived cones remain to be defined, in addition to development of cone cell purification strategies. Here, we report an analysis of the human L/M-opsin cone photoreceptor transcriptome using an AAV2/9.pR2.1:GFP reporter. This led to the identification of a cone-enriched gene signature, which we used to demonstrate similar gene expression between fetal and stem cell-derived cones. We then defined a cluster of differentiation marker combination that, when used for cell sorting, significantly enriches for cone photoreceptors from the fetal retina and stem cell-derived retinal organoids, respectively. These data may facilitate more efficient isolation of human stem cell-derived cones for use in clinical transplantation studies.
Collections
Subject
Retina
Fetus
Humans
Retinal Degeneration
Gene Expression Profiling
Cell Differentiation
Gene Expression Regulation, Developmental
Rod Opsins
Retinal Cone Photoreceptor Cells
Induced Pluripotent Stem Cells
Transcriptome
Research team
Translational Genomics
Language
eng
Date accepted
2017-10-15
License start date
2017-12
Citation
Stem cell reports, 2017, 9 (6), pp. 1898 - 1915