Browsing ICR Divisions by author "Bakal, Christopher"
Now showing items 21-34 of 34
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Microenvironmental Heterogeneity Parallels Breast Cancer Progression: A Histology-Genomic Integration Analysis.
Natrajan, R; Sailem, H; Mardakheh, FK; Arias Garcia, M; Tape, CJ; et al. (PUBLIC LIBRARY SCIENCE, 2016-02-16)BACKGROUND: The intra-tumor diversity of cancer cells is under intense investigation; however, little is known about the heterogeneity of the tumor microenvironment that is key to cancer progression and evolution. We aimed ... -
Multiparametric Analysis of Cell Shape Demonstrates that β-PIX Directly Couples YAP Activation to Extracellular Matrix Adhesion.
Sero, JE; Bakal, C (CELL PRESS, 2017-01-25)Mechanical signals from the extracellular matrix (ECM) and cellular geometry regulate the nuclear translocation of transcriptional regulators such as Yes-associated protein (YAP). Elucidating how physical signals control ... -
Nanoneedle-Mediated Stimulation of Cell Mechanotransduction Machinery.
Hansel, CS; Crowder, SW; Cooper, S; Gopal, S; João Pardelha da Cruz, M; et al. (AMER CHEMICAL SOC, 2019-03-26)Biomaterial substrates can be engineered to present topographical signals to cells which, through interactions between the material and active components of the cell membrane, regulate key cellular processes and guide cell ... -
NucliTrack: an integrated nuclei tracking application.
Cooper, S; Barr, AR; Glen, R; Bakal, C (OXFORD UNIV PRESS, 2017-10-15)SUMMARY: Live imaging studies give unparalleled insight into dynamic single cell behaviours and fate decisions. However, the challenge of reliably tracking single cells over long periods of time limits both the throughput ... -
Proteomic characterisation of triple negative breast cancer cells following CDK4/6 inhibition.
Beykou, M; Arias-Garcia, M; Roumeliotis, TI; Choudhary, JS; Moser, N; et al. (NATURE PORTFOLIO, 2022-07-11)When used in combination with hormone treatment, Palbociclib prolongs progression-free survival of patients with hormone receptor positive breast cancer. Mechanistically, Palbociclib inhibits CDK4/6 activity but the basis ... -
Proteomics profiling of interactome dynamics by colocalisation analysis (COLA).
Mardakheh, FK; Sailem, HZ; Kümper, S; Tape, CJ; McCully, RR; et al. (ROYAL SOC CHEMISTRY, 2016-12-20)Localisation and protein function are intimately linked in eukaryotes, as proteins are localised to specific compartments where they come into proximity of other functionally relevant proteins. Significant co-localisation ... -
Quantitative imaging of single-cell phenotypes in cancer cells cultured on hydrogel surfaces.
Bousgouni, V; Bakal, C (ELSEVIER, 2023-03-17)Small interfering RNA (siRNA) screening approaches used with quantitative single-cell analysis can uncover the roles of genes in cell morphogenesis. Here, we present a high-throughput automated phenotypic screening technique ... -
RNAi screens for Rho GTPase regulators of cell shape and YAP/TAZ localisation in triple negative breast cancer.
Pascual-Vargas, P; Cooper, S; Sero, J; Bousgouni, V; Arias-Garcia, M; et al. (NATURE PUBLISHING GROUP, 2017-03-01)In order to metastasise, triple negative breast cancer (TNBC) must make dynamic changes in cell shape. The shape of all eukaryotic cells is regulated by Rho Guanine Nucleotide Exchange Factors (RhoGEFs), which activate ... -
Robust deep learning optical autofocus system applied to automated multiwell plate single molecule localization microscopy.
Lightley, J; Görlitz, F; Kumar, S; Kalita, R; Kolbeinsson, A; et al. (WILEY, 2021-06-04)We presenta robust, long-range optical autofocus system for microscopy utilizing machine learning. This can be useful for experiments with long image data acquisition times that may be impacted by defocusing resulting from ... -
Single-Cell Dynamics Determines Response to CDK4/6 Inhibition in Triple-Negative Breast Cancer.
Asghar, US; Barr, AR; Cutts, R; Beaney, M; Babina, I; et al. (AMER ASSOC CANCER RESEARCH, 2017-09-15)Purpose: Triple-negative breast cancer (TNBC) is a heterogeneous subgroup of breast cancer that is associated with a poor prognosis. We evaluated the activity of CDK4/6 inhibitors across the TNBC subtypes and investigated ... -
Squeezing through the microcirculation: survival adaptations of circulating tumour cells to seed metastasis.
Perea Paizal, J; Au, SH; Bakal, C (SPRINGERNATURE, 2021-01-05)During metastasis, tumour cells navigating the vascular circulatory system-circulating tumour cells (CTCs)-encounter capillary beds, where they start the process of extravasation. Biomechanical constriction forces exerted ... -
Visualizing cellular imaging data using PhenoPlot.
Sailem, HZ; Sero, JE; Bakal, C (NATURE PUBLISHING GROUP, 2015-01-08)Visualization is essential for data interpretation, hypothesis formulation and communication of results. However, there is a paucity of visualization methods for image-derived data sets generated by high-content analysis ... -
Voltage imaging reveals the dynamic electrical signatures of human breast cancer cells.
Quicke, P; Sun, Y; Arias-Garcia, M; Beykou, M; Acker, CD; et al. (NATURE PORTFOLIO, 2022-11-11)Cancer cells feature a resting membrane potential (Vm) that is depolarized compared to normal cells, and express active ionic conductances, which factor directly in their pathophysiological behavior. Despite similarities ... -
WDR5, BRCA1, and BARD1 Co-regulate the DNA Damage Response and Modulate the Mesenchymal-to-Epithelial Transition during Early Reprogramming.
Peñalosa-Ruiz, G; Bousgouni, V; Gerlach, JP; Waarlo, S; van de Ven, JV; et al. (CELL PRESS, 2019-04-09)Differentiated cells are epigenetically stable, but can be reprogrammed to pluripotency by expression of the OSKM transcription factors. Despite significant effort, relatively little is known about the cellular requirements ...