Proteomics profiling of interactome dynamics by colocalisation analysis (COLA).
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Date
2016-12-20Author
Mardakheh, FK
Sailem, HZ
Kümper, S
Tape, CJ
McCully, RR
Paul, A
Anjomani-Virmouni, S
Jørgensen, C
Poulogiannis, G
Marshall, CJ
Bakal, C
Type
Journal Article
Metadata
Show full item recordAbstract
Localisation and protein function are intimately linked in eukaryotes, as proteins are localised to specific compartments where they come into proximity of other functionally relevant proteins. Significant co-localisation of two proteins can therefore be indicative of their functional association. We here present COLA, a proteomics based strategy coupled with a bioinformatics framework to detect protein-protein co-localisations on a global scale. COLA reveals functional interactions by matching proteins with significant similarity in their subcellular localisation signatures. The rapid nature of COLA allows mapping of interactome dynamics across different conditions or treatments with high precision.
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Subject
Cell Line
Intracellular Space
Subcellular Fractions
Humans
Proteome
Chromatography, Liquid
Cell Fractionation
Cluster Analysis
Sensitivity and Specificity
Protein Interaction Mapping
Proteomics
Protein Binding
Protein Transport
Mass Spectrometry
Protein Interaction Maps
Research team
Dynamical Cell Systems
Signalling & Cancer Metabolism
Oncogene
Language
eng
Date accepted
2016-11-01
License start date
2016-12
Citation
Molecular bioSystems, 2016, 13 (1), pp. 92 - 105
Publisher
ROYAL SOC CHEMISTRY