Nanoneedle-Mediated Stimulation of Cell Mechanotransduction Machinery.
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Date
2019-03-26ICR Author
Author
Hansel, CS
Crowder, SW
Cooper, S
Gopal, S
João Pardelha da Cruz, M
de Oliveira Martins, L
Keller, D
Rothery, S
Becce, M
Cass, AEG
Bakal, C
Chiappini, C
Stevens, MM
Type
Journal Article
Metadata
Show full item recordAbstract
Biomaterial substrates can be engineered to present topographical signals to cells which, through interactions between the material and active components of the cell membrane, regulate key cellular processes and guide cell fate decisions. However, targeting mechanoresponsive elements that reside within the intracellular domain is a concept that has only recently emerged. Here, we show that mesoporous silicon nanoneedle arrays interact simultaneously with the cell membrane, cytoskeleton, and nucleus of primary human cells, generating distinct responses at each of these cellular compartments. Specifically, nanoneedles inhibit focal adhesion maturation at the membrane, reduce tension in the cytoskeleton, and lead to remodeling of the nuclear envelope at sites of impingement. The combined changes in actin cytoskeleton assembly, expression and segregation of the nuclear lamina, and localization of Yes-associated protein (YAP) correlate differently from what is canonically observed upon stimulation at the cell membrane, revealing that biophysical cues directed to the intracellular space can generate heretofore unobserved mechanosensory responses. These findings highlight the ability of nanoneedles to study and direct the phenotype of large cell populations simultaneously, through biophysical interactions with multiple mechanoresponsive components.
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Subject
Cells, Cultured
Humans
Silicon
Needles
Cell Adhesion
Mechanotransduction, Cellular
Particle Size
Surface Properties
Porosity
Nanostructures
Human Umbilical Vein Endothelial Cells
Research team
Dynamical Cell Systems
Language
eng
License start date
2019-03-04
Citation
ACS nano, 2019, 13 (3), pp. 2913 - 2926
Publisher
AMER CHEMICAL SOC