Impact of mutational profiles on response of primary oestrogen receptor-positive breast cancers to oestrogen deprivation.
POETIC Trial Management Group and Trialists
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Pre-surgical studies allow study of the relationship between mutations and response of oestrogen receptor-positive (ER+) breast cancer to aromatase inhibitors (AIs) but have been limited to small biopsies. Here in phase I of this study, we perform exome sequencing on baseline, surgical core-cuts and blood from 60 patients (40 AI treated, 20 controls). In poor responders (based on Ki67 change), we find significantly more somatic mutations than good responders. Subclones exclusive to baseline or surgical cores occur in ∼30% of tumours. In phase II, we combine targeted sequencing on another 28 treated patients with phase I. We find six genes frequently mutated: PIK3CA, TP53, CDH1, MLL3, ABCA13 and FLG with 71% concordance between paired cores. TP53 mutations are associated with poor response. We conclude that multiple biopsies are essential for confident mutational profiling of ER+ breast cancer and TP53 mutations are associated with resistance to oestrogen deprivation therapy.
Version of record
Aged, 80 and over
DNA Mutational Analysis
Drug Resistance, Neoplasm
Tumor Suppressor Protein p53
Whole Exome Sequencing
Clinical Trials & Statistics Unit
Medicine (RMH Smith Cunningham)
License start date
Nat Commun, 7 pp. 13294 - ?