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dc.contributor.authorCroft, DR
dc.contributor.authorColeman, ML
dc.contributor.authorLi, S
dc.contributor.authorRobertson, D
dc.contributor.authorSullivan, T
dc.contributor.authorStewart, CL
dc.contributor.authorOlson, MF
dc.date.accessioned2018-06-11T13:38:48Z
dc.date.issued2005-01
dc.identifier.citationThe Journal of cell biology, 2005, 168 (2), pp. 245 - 255
dc.identifier.issn0021-9525
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/1825
dc.identifier.eissn1540-8140
dc.identifier.doi10.1083/jcb.200409049
dc.description.abstractMembrane blebbing during the apoptotic execution phase results from caspase-mediated cleavage and activation of ROCK I. Here, we show that ROCK activity, myosin light chain (MLC) phosphorylation, MLC ATPase activity, and an intact actin cytoskeleton, but not microtubular cytoskeleton, are required for disruption of nuclear integrity during apoptosis. Inhibition of ROCK or MLC ATPase activity, which protect apoptotic nuclear integrity, does not affect caspase-mediated degradation of nuclear proteins such as lamins A, B1, or C. The conditional activation of ROCK I was sufficient to tear apart nuclei in lamin A/C null fibroblasts, but not in wild-type fibroblasts. Thus, apoptotic nuclear disintegration requires actin-myosin contractile force and lamin proteolysis, making apoptosis analogous to, but distinct from, mitosis where nuclear disintegration results from microtubule-based forces and from lamin phosphorylation and depolymerization.
dc.formatPrint
dc.format.extent245 - 255
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0
dc.subjectNIH 3T3 Cells
dc.subjectNuclear Lamina
dc.subjectCell Nucleus
dc.subjectCytoskeleton
dc.subjectMicrotubules
dc.subjectFibroblasts
dc.subjectAnimals
dc.subjectMice
dc.subjectAmides
dc.subjectCycloheximide
dc.subjectPyridines
dc.subjectNocodazole
dc.subjectCytochalasin D
dc.subjectActins
dc.subjectMyosins
dc.subjectMyosin-Light-Chain Phosphatase
dc.subjectCaspases
dc.subjectProtein Kinases
dc.subjectProtein-Serine-Threonine Kinases
dc.subjectTumor Necrosis Factor-alpha
dc.subjectIntracellular Signaling Peptides and Proteins
dc.subjectMyosin Light Chains
dc.subjectCytoskeletal Proteins
dc.subjectNuclear Proteins
dc.subjectLamins
dc.subjectPhosphoproteins
dc.subjectEnzyme Inhibitors
dc.subjectMicroscopy, Electron, Transmission
dc.subjectTransfection
dc.subjectApoptosis
dc.subjectPhosphorylation
dc.subjectMutation
dc.subjectrho-Associated Kinases
dc.subjectLim Kinases
dc.subjectCaspase Inhibitors
dc.titleActin-myosin-based contraction is responsible for apoptotic nuclear disintegration.
dc.typeJournal Article
rioxxterms.versionofrecord10.1083/jcb.200409049
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc-sa/4.0
rioxxterms.licenseref.startdate2005-01
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfThe Journal of cell biology
pubs.issue2
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research/Genetic Susceptibility
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Genetics and Epidemiology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Genetics and Epidemiology/Genetic Susceptibility
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research/Genetic Susceptibility
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Genetics and Epidemiology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Genetics and Epidemiology/Genetic Susceptibility
pubs.publication-statusPublished
pubs.volume168
pubs.embargo.termsNot known
icr.researchteamGenetic Susceptibilityen_US
dc.contributor.icrauthorStratton, Michaelen


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