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dc.contributor.authorSarrio, D
dc.contributor.authorPalacios, J
dc.contributor.authorHergueta-Redondo, M
dc.contributor.authorGomez-Lopez, G
dc.contributor.authorCano, A
dc.contributor.authorMoreno-Bueno, G
dc.identifier.citationBMC CANCER, 2009, 9
dc.description.abstractBackground: Alterations in the cadherin-catenin adhesion complexes are involved in tumor initiation, progression and metastasis. However, the functional implication of distinct cadherin types in breast cancer biology is still poorly understood. Methods: To compare the functional role of E-cadherin and P-cadherin in invasive breast cancer, we stably transfected these molecules into the MDA-MB-231 cell line, and investigated their effects on motility, invasion and gene expression regulation. Results: Expression of either E-and P-cadherin significantly increased cell aggregation and induced a switch from fibroblastic to epithelial morphology. Although expression of these cadherins did not completely reverse the mesenchymal phenotype of MDA-MB-231 cells, both E-and P-cadherin decreased fibroblast-like migration and invasion through extracellular matrix in a similar way. Moreover, microarray gene expression analysis of MDA-MB-231 cells after expression of E-and P-cadherins revealed that these molecules can activate signaling pathways leading to significant changes in gene expression. Although the expression patterns induced by E-and P-cadherin showed more similarities than differences, 40 genes were differentially modified by the expression of either cadherin type. Conclusion: E-and P-cadherin have similar functional consequences on the phenotype and invasive behavior of MDA-MB-231 cells. Moreover, we demonstrate for the first time that these cadherins can induce both common and specific gene expression programs on invasive breast cancer cells. Importantly, these identified genes are potential targets for future studies on the functional consequences of altered cadherin expression in human breast cancer.
dc.titleFunctional characterization of E- and P-cadherin in invasive breast cancer cells
dc.typeJournal Article
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfBMC CANCER
pubs.notesaffiliation: Moreno-Bueno, G (Reprint Author), CSIC UAM, Inst Invest Biomed Alberto Sols, Dept Biochem, C Arturo Duperier 4, Madrid 28029, Spain. Sarrio, David; Hergueta-Redondo, Marta; Cano, Amparo; Moreno-Bueno, Gema, CSIC UAM, Inst Invest Biomed Alberto Sols, Dept Biochem, Madrid 28029, Spain. Sarrio, David, Inst Canc Res, Breakthrough Breast Canc Res Ctr, London SW3 6JB, England. Palacios, Jose, Hosp Virgen Rocio, Serv Anat Patol, Seville 41013, Spain. Gomez-Lopez, Gonzalo, CNIO, Spanish Natl Canc Res Ctr, Bioinformat Unit, UBio, Madrid 28029, Spain. article-number: 74 keywords-plus: GENE-EXPRESSION; TUMOR PROGRESSION; CARCINOMA-CELLS; DOWN-REGULATION; ADHESION; PROMOTES; SUPPRESSION; MOTILITY; COMPLEX; P120CTN research-areas: Oncology web-of-science-categories: Oncology author-email: [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] researcherid-numbers: Gomez-Lopez, Gonzalo/D-9123-2016 Moreno-Bueno, Gema/K-9354-2016 orcid-numbers: Gomez-Lopez, Gonzalo/0000-0002-4146-0551 Moreno-Bueno, Gema/0000-0002-5030-6687 funding-acknowledgement: Fundacion Mutua Madrile; European Commission [PIEF-GA-2008-221083]; [SAF2007-63075]; [SAF 200763051]; [PI051890]; [SAF2004-08258-C02-01] funding-text: The authors thank to Drs Albert Reynolds (Nashville, USA), Keith R Johnson (Omaha, USA), and Setsuo Hirohashi (Tokyo, Japan) for providing cDNAs and plasmids. We are also grateful to Diego Meg as (CNIO, Spain) and Dr Ester Mart n-Villar (CSIC, Spain) for helping with cell migration and invasion assays. This work has been supported by grants from the Fundacion Mutua Madrile a (2006) and SAF2007-63075 to GMB; SAF 200763051 to AC; PI051890 and SAF2004-08258-C02-01 to JP. David Sarrio currently is a recipient of a Marie Curie Intra-European Fellowship of the European Commission (PIEF-GA-2008-221083). Gema Moreno-Bueno is a junior investigator of the “Ramon y Cajal Program” of the Spanish Ministry of Education and Science (2004). number-of-cited-references: 49 times-cited: 41 usage-count-last-180-days: 0 usage-count-since-2013: 14 journal-iso: BMC Cancer doc-delivery-number: 429IP unique-id: ISI:000264914400001 oa: gold da: 2018-08-29
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dc.contributor.icrauthorSarrio, Daviden

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