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dc.contributor.authorNeidle, S
dc.date.accessioned2018-09-25T10:36:40Z
dc.date.issued2001-05-01
dc.identifierhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC37244/
dc.identifier.citationNUCLEIC ACIDS RESEARCH, 2001, 29 (9), pp. 1935 - 1942
dc.identifier.issn0305-1048
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2864
dc.description.abstractStabilisation of TG-and AG-containing antiparrallel DNA triplexes by triplex-binding ligands. We have used DNase I footprinting to examine the interaction of several tripler-binding ligands with antiparallel TG- and AG- containing triplexes. We find that although a 17mer TG- containing oligonucleotide on its own fails to produce a footprint at concentrations as high as 30 CIM, this interaction can be stabilised by several ligands. Within a series of disubstituted amidoanthraquinones we find that the 2,7- regioisomer affords the best stabilisation of this TG tripler, though the 1,8- isomer also stabilises this interaction to some extent. By contrast the 1,5- and 2,6- regioisomers show no interaction with TG triplexes. Similar studies with a 13mer AG- containing oligonucleotide show the opposite pattern of stabilisation: the 2,6- and 1,5- isomers stabilise this tripler, but the 2,7- and 1,8-compounds do not. The polycyclic compound BePI strongly stabilises TG- but not AG-containing triplexes, while a substituted naphthylquinoline interacts with both antiparallel tripler motifs.
dc.format.extent1935 - 1942
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://www.rioxx.net/licenses/all-rights-reserved
dc.subjectACRIDINE-LINKED OLIGONUCLEOTIDES; CENTER-DOT-PYRIMIDINE; HELIX FORMATION; BASE TRIPLE; PURINE-RICH; HUMAN TELOMERASE; STABILIZATION; OLIGODEOXYNUCLEOTIDES; INHIBITION; CORALYNE
dc.titleStabilisation of TG-and AG-containing antiparrallel DNA triplexes by triplex-binding ligands
dc.typeJournal Article
rioxxterms.licenseref.urihttps://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2001-05-01
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfNUCLEIC ACIDS RESEARCH
pubs.issue9
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR
pubs.volume29
pubs.embargo.termsNot known
dc.contributor.icrauthorNeidle, Stephenen


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