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dc.contributor.authorCocker, HAen_US
dc.contributor.authorHobbs, SMen_US
dc.contributor.authorTiffin, Nen_US
dc.contributor.authorPritchard-Jones, Ken_US
dc.contributor.authorPinkerton, CRen_US
dc.contributor.authorKelland, LRen_US
dc.date.accessioned2018-09-26T08:32:41Z
dc.date.issued2001-11-30en_US
dc.identifier11en_US
dc.identifier.citationBRITISH JOURNAL OF CANCER, 2001, 85 pp. 1746 - 1752en_US
dc.identifier.issn0007-0920en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2867
dc.identifier.doi10.1054/bjoc.2001.2145en_US
dc.description.abstractThe MDM2 protein is known to be overexpressed in some sarcomas including rhabdomyosarcoma. However, the extent to which the MDM2 protein influences sensitivity to chemotherapeutic drugs is unclear. We have analysed this further using stable transfection of the mdm2 gene into 4 well-characterised human paediatric rhabdomyosarcoma cell lines. Transfection with the mdm2 gene resulted in increased levels of the MDM2 protein in all the cell lines. In 2 of the lines. SCMC and RD, the mdm2 gene caused between 2-fold and 61-fold increase in resistance to vincristine, etoposide and doxorubicin but not to cisplatin. In these lines there was an increase in expression of the mdr-1 gene which encodes P-glycoprotein, but not the mrp1 gene which encodes the multidrug resistance protein (MRP). The resistance was reversible using the MDR modulator PSC833. confirming the presence of P-glycoprotein. We conclude that MDM2 overexpression may be a mechanism by which multidrug resistance is regulated in some rhabdomyosarcomas. (C) 2001 Cancer Research Campaign.en_US
dc.format.extent1746 - 1752en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.titleHigh levels of the MDM2 oncogene in paediatric rhabdomyosarcoma cell lines may confer multidrug resistanceen_US
dc.typeJournal Article
rioxxterms.versionofrecord10.1054/bjoc.2001.2145en_US
rioxxterms.licenseref.startdate2001-11-30en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfBRITISH JOURNAL OF CANCERen_US
pubs.notesresearcherid-numbers: pinkerton, ross/I-4808-2014 Tiffin, Nicki/A-5914-2013 Pritchard-Jones, Kathy/F-4286-2014 orcid-numbers: Pritchard-Jones, Kathy/0000-0002-2384-9475 unique-id: ISI:000173054800022en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.volume85en_US
pubs.embargo.termsNot knownen_US
dc.contributor.icrauthorPritchard-Jones, Kathyen_US
dc.contributor.icrauthorKelland, Lloyden_US
dc.contributor.icrauthorPinkerton, Rossen_US


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