Now showing items 1-9 of 9

    • Activation of the Aryl Hydrocarbon Receptor Interferes with Early Embryonic Development. 

      Gialitakis, M; Tolaini, M; Li, Y; Pardo, M; Yu, L; Toribio, A; Choudhary, JS; Niakan, K; Papayannopoulos, V; Stockinger, B (2017-11)
      The transcriptional program of early embryonic development is tightly regulated by a set of well-defined transcription factors that suppress premature expression of differentiation genes and sustain the pluripotent identity. ...
    • The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation. 

      Zhang, W; Chronis, C; Chen, X; Zhang, H; Spalinskas, R; Pardo, M; Chen, L; Wu, G; Zhu, Z; Yu, Y; Yu, L; Choudhary, J; Nichols, J; Parast, MM; Greber, B; Sahlén, P; Plath, K (2019-01)
      BAF complexes are composed of different subunits with varying functional and developmental roles, although many subunits have not been examined in depth. Here we show that the Baf45 subunit Dpf2 maintains pluripotency and ...
    • C9orf72 arginine-rich dipeptide proteins interact with ribosomal proteins in vivo to induce a toxic translational arrest that is rescued by eIF1A. 

      Moens, TG; Niccoli, T; Wilson, KM; Atilano, ML; Birsa, N; Gittings, LM; Holbling, BV; Dyson, MC; Thoeng, A; Neeves, J; Glaria, I; Yu, L; Bussmann, J; Storkebaum, E; Pardo, M; Choudhary, JS; Fratta, P; Partridge, L; Isaacs, AM (2019-03)
      A GGGGCC hexanucleotide repeat expansion within the C9orf72 gene is the most common genetic cause of both amyotrophic lateral sclerosis and frontotemporal dementia. Sense and antisense repeat-containing transcripts undergo ...
    • Characterization of Two Distinct Nucleosome Remodeling and Deacetylase (NuRD) Complex Assemblies in Embryonic Stem Cells. 

      Bode, D; Yu, L; Tate, P; Pardo, M; Choudhary, J (2016-03)
      Pluripotency and self-renewal, the defining properties of embryonic stem cells, are brought about by transcriptional programs involving an intricate network of transcription factors and chromatin remodeling complexes. The ...
    • Cyclin B1-Cdk1 facilitates MAD1 release from the nuclear pore to ensure a robust spindle checkpoint. 

      Jackman, M; Marcozzi, C; Barbiero, M; Pardo, M; Yu, L; Tyson, AL; Choudhary, JS; Pines, J (2020-06)
      How the cell rapidly and completely reorganizes its architecture when it divides is a problem that has fascinated researchers for almost 150 yr. We now know that the core regulatory machinery is highly conserved in eukaryotes, ...
    • Genomic Determinants of Protein Abundance Variation in Colorectal Cancer Cells. 

      Roumeliotis, TI; Williams, SP; Gonçalves, E; Alsinet, C; Del Castillo Velasco-Herrera, M; Aben, N; Ghavidel, FZ; Michaut, M; Schubert, M; Price, S; Wright, JC; Yu, L; Yang, M; Dienstmann, R; Guinney, J; Beltrao, P; Brazma, A; Pardo, M; Stegle, O; Adams, DJ; Wessels, L; Saez-Rodriguez, J; McDermott, U; Choudhary, JS (2017-08)
      Assessing the impact of genomic alterations on protein networks is fundamental in identifying the mechanisms that shape cancer heterogeneity. We have used isobaric labeling to characterize the proteomic landscapes of 50 ...
    • Landscape of the Plasmodium Interactome Reveals Both Conserved and Species-Specific Functionality. 

      Hillier, C; Pardo, M; Yu, L; Bushell, E; Sanderson, T; Metcalf, T; Herd, C; Anar, B; Rayner, JC; Billker, O; Choudhary, JS (2019-08)
      Malaria represents a major global health issue, and the identification of new intervention targets remains an urgent priority. This search is hampered by more than one-third of the genes of malaria-causing Plasmodium ...
    • Mutations in FAM50A suggest that Armfield XLID syndrome is a spliceosomopathy. 

      Lee, Y-R; Khan, K; Armfield-Uhas, K; Srikanth, S; Thompson, NA; Pardo, M; Yu, L; Norris, JW; Peng, Y; Gripp, KW; Aleck, KA; Li, C; Spence, E; Choi, T-I; Kwon, SJ; Park, H-M; Yu, D; Do Heo, W; Mooney, MR; Baig, SM; Wentzensen, IM; Telegrafi, A; McWalter, K; Moreland, T; Roadhouse, C; Ramsey, K; Lyons, MJ; Skinner, C; Alexov, E; Katsanis, N; Stevenson, RE; Choudhary, JS; Adams, DJ; Kim, C-H; Davis, EE; Schwartz, CE (2020-07-23)
      Intellectual disability (ID) is a heterogeneous clinical entity and includes an excess of males who harbor variants on the X-chromosome (XLID). We report rare FAM50A missense variants in the original Armfield XLID syndrome ...
    • Proteomic navigation using proximity-labeling. 

      Gentzel, M; Pardo, M; Subramaniam, S; Stewart, AF; Choudhary, JS (2019-07)
      The identification of bona fide protein-protein interactions and the mapping of proteomes was greatly enhanced by protein tagging for generic affinity purification methods and analysis by mass spectrometry (AP-MS). The ...