Browsing Cancer Biology by author "Pardo Calvo, Maria Mercedes"
Now showing items 1-20 of 29
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A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component
Cutts, E; Taylor, G; Pardo, M; Yu, L; Wills, J; et al. (2021-01-13)Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. ... -
A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component.
Cutts, EE; Taylor, GC; Pardo, M; Yu, L; Wills, JC; et al. (F1000 Research Ltd, 2021-01-13)Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. ... -
Activation of the Aryl Hydrocarbon Receptor Interferes with Early Embryonic Development.
Gialitakis, M; Tolaini, M; Li, Y; Pardo, M; Yu, L; et al. (CELL PRESS, 2017-10-01)The transcriptional program of early embryonic development is tightly regulated by a set of well-defined transcription factors that suppress premature expression of differentiation genes and sustain the pluripotent identity. ... -
Aneuploidy tolerance caused by BRG1 loss allows chromosome gains and recovery of fitness.
Schiavoni, F; Zuazua-Villar, P; Roumeliotis, TI; Benstead-Hume, G; Pardo, M; et al. (NATURE PORTFOLIO, 2022-04-01)Aneuploidy results in decreased cellular fitness in many species and model systems. However, aneuploidy is commonly found in cancer cells and often correlates with aggressive growth, suggesting that the impact of aneuploidy ... -
C9orf72 arginine-rich dipeptide proteins interact with ribosomal proteins in vivo to induce a toxic translational arrest that is rescued by eIF1A.
Moens, TG; Niccoli, T; Wilson, KM; Atilano, ML; Birsa, N; et al. (SPRINGER, 2019-03-01)A GGGGCC hexanucleotide repeat expansion within the C9orf72 gene is the most common genetic cause of both amyotrophic lateral sclerosis and frontotemporal dementia. Sense and antisense repeat-containing transcripts undergo ... -
Candida albicans Hyphal Extracellular Vesicles Are Different from Yeast Ones, Carrying an Active Proteasome Complex and Showing a Different Role in Host Immune Response.
Martínez-López, R; Hernáez, ML; Redondo, E; Calvo, G; Radau, S; et al. (AMER SOC MICROBIOLOGY, 2022-06-29)Candida albicans is the principal causative agent of lethal fungal infections, predominantly in immunocompromised hosts. Extracellular vesicles (EVs) have been described as crucial in the interaction of microorganisms with ... -
Characterization of Two Distinct Nucleosome Remodeling and Deacetylase (NuRD) Complex Assemblies in Embryonic Stem Cells.
Bode, D; Yu, L; Tate, P; Pardo, M; Choudhary, J (AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2016-03-01)Pluripotency and self-renewal, the defining properties of embryonic stem cells, are brought about by transcriptional programs involving an intricate network of transcription factors and chromatin remodeling complexes. The ... -
CRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisation.
van der Weyden, L; Harle, V; Turner, G; Offord, V; Iyer, V; et al. (NATURE PORTFOLIO, 2021-03-23)Melanoma represents ~5% of all cutaneous malignancies, yet accounts for the majority of skin cancer deaths due to its propensity to metastasise. To develop new therapies, novel target molecules must to be identified and ... -
Cyclin B1-Cdk1 facilitates MAD1 release from the nuclear pore to ensure a robust spindle checkpoint.
Jackman, M; Marcozzi, C; Barbiero, M; Pardo, M; Yu, L; et al. (ROCKEFELLER UNIV PRESS, 2020-06-01)How the cell rapidly and completely reorganizes its architecture when it divides is a problem that has fascinated researchers for almost 150 yr. We now know that the core regulatory machinery is highly conserved in eukaryotes, ... -
Delineating the HMGB1 and HMGB2 interactome in prostate and ovary epithelial cells and its relationship with cancer.
Barreiro-Alonso, A; Lamas-Maceiras, M; García-Díaz, R; Rodríguez-Belmonte, E; Yu, L; et al. (Impact Journals, LLC, 2018-04-10)High Mobility Group B (HMGB) proteins are involved in cancer progression and in cellular responses to platinum compounds used in the chemotherapy of prostate and ovary cancer. Here we use affinity purification coupled to ... -
Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity.
Thomas, DC; Clare, S; Sowerby, JM; Pardo, M; Juss, JK; et al. (ROCKEFELLER UNIV PRESS, 2017-04-03)The phagocyte respiratory burst is crucial for innate immunity. The transfer of electrons to oxygen is mediated by a membrane-bound heterodimer, comprising gp91phox and p22phox subunits. Deficiency of either subunit leads ... -
EROS is a selective chaperone regulating the phagocyte NADPH oxidase and purinergic signalling.
Randzavola, LO; Mortimer, PM; Garside, E; Dufficy, ER; Schejtman, A; et al. (eLIFE SCIENCES PUBL LTD, 2022-11-24)EROS (essential for reactive oxygen species) protein is indispensable for expression of gp91phox, the catalytic core of the phagocyte NADPH oxidase. EROS deficiency in humans is a novel cause of the severe immunodeficiency, ... -
Genomic Determinants of Protein Abundance Variation in Colorectal Cancer Cells.
Roumeliotis, TI; Williams, SP; Gonçalves, E; Alsinet, C; Del Castillo Velasco-Herrera, M; et al. (CELL PRESS, 2017-08-29)Assessing the impact of genomic alterations on protein networks is fundamental in identifying the mechanisms that shape cancer heterogeneity. We have used isobaric labeling to characterize the proteomic landscapes of 50 ... -
HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes.
Barreiro-Alonso, A; Lamas-Maceiras, M; Lorenzo-Catoira, L; Pardo, M; Yu, L; et al. (MDPI, 2021-09-18)This study reports the HMGB1 interactomes in prostate and ovary cancer cells lines. Affinity purification coupled to mass spectrometry confirmed that the HMGB1 nuclear interactome is involved in HMGB1 known functions such ... -
Identifying and characterising Thrap3, Bclaf1 and Erh interactions using cross-linking mass spectrometry
Shcherbakova, L; Pardo, M; Roumeliotis, T; Choudhary, J (F1000 Research Ltd, 2023-01-06)<ns3:p><ns3:bold>Background: </ns3:bold>Cross-linking mass spectrometry (XL-MS) is a powerful technology capable of yielding structural insights across the complex cellular protein interaction network. However, up to date ... -
Identifying and characterising Thrap3, Bclaf1 and Erh interactions using cross-linking mass spectrometry.
Shcherbakova, L; Pardo, M; Roumeliotis, T; Choudhary, J (F1000 Research Ltd, 2021-01-01)Background: Cross-linking mass spectrometry (XL-MS) is a powerful technology capable of yielding structural insights across the complex cellular protein interaction network. However, up to date most of the studies utilising ... -
Integrative epigenomics, transcriptomics and proteomics of patient chondrocytes reveal genes and pathways involved in osteoarthritis.
Steinberg, J; Ritchie, GRS; Roumeliotis, TI; Jayasuriya, RL; Clark, MJ; et al. (NATURE PORTFOLIO, 2017-08-21)Osteoarthritis (OA) is a common disease characterized by cartilage degeneration and joint remodeling. The underlying molecular changes underpinning disease progression are incompletely understood. We investigated genes and ... -
Landscape of the Plasmodium Interactome Reveals Both Conserved and Species-Specific Functionality.
Hillier, C; Pardo, M; Yu, L; Bushell, E; Sanderson, T; et al. (CELL PRESS, 2019-08-06)Malaria represents a major global health issue, and the identification of new intervention targets remains an urgent priority. This search is hampered by more than one-third of the genes of malaria-causing Plasmodium ... -
Multiple short windows of calcium-dependent protein kinase 4 activity coordinate distinct cell cycle events during Plasmodium gametogenesis.
Fang, H; Klages, N; Baechler, B; Hillner, E; Yu, L; et al. (ELIFE SCIENCES PUBLICATIONS LTD, 2017-05-08)Malaria transmission relies on the production of gametes following ingestion by a mosquito. Here, we show that Ca2+-dependent protein kinase 4 controls three processes essential to progress from a single haploid microgametocyte ... -
Mutations in FAM50A suggest that Armfield XLID syndrome is a spliceosomopathy.
Lee, Y-R; Khan, K; Armfield-Uhas, K; Srikanth, S; Thompson, NA; et al. (NATURE PORTFOLIO, 2020-07-23)Intellectual disability (ID) is a heterogeneous clinical entity and includes an excess of males who harbor variants on the X-chromosome (XLID). We report rare FAM50A missense variants in the original Armfield XLID syndrome ...