Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity.
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Date
2017-04-03Author
Thomas, DC
Clare, S
Sowerby, JM
Pardo, M
Juss, JK
Goulding, DA
van der Weyden, L
Storisteanu, D
Prakash, A
Espéli, M
Flint, S
Lee, JC
Hoenderdos, K
Kane, L
Harcourt, K
Mukhopadhyay, S
Umrania, Y
Antrobus, R
Nathan, JA
Adams, DJ
Bateman, A
Choudhary, JS
Lyons, PA
Condliffe, AM
Chilvers, ER
Dougan, G
Smith, KGC
Type
Journal Article
Metadata
Show full item recordAbstract
The phagocyte respiratory burst is crucial for innate immunity. The transfer of electrons to oxygen is mediated by a membrane-bound heterodimer, comprising gp91phox and p22phox subunits. Deficiency of either subunit leads to severe immunodeficiency. We describe Eros (essential for reactive oxygen species), a protein encoded by the previously undefined mouse gene bc017643, and show that it is essential for host defense via the phagocyte NAPDH oxidase. Eros is required for expression of the NADPH oxidase components, gp91phox and p22phox Consequently, Eros-deficient mice quickly succumb to infection. Eros also contributes to the formation of neutrophil extracellular traps (NETS) and impacts on the immune response to melanoma metastases. Eros is an ortholog of the plant protein Ycf4, which is necessary for expression of proteins of the photosynthetic photosystem 1 complex, itself also an NADPH oxio-reductase. We thus describe the key role of the previously uncharacterized protein Eros in host defense.
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Subject
Neutrophils
Endoplasmic Reticulum
Macrophages
Phagocytes
Animals
Mice, Inbred C57BL
Humans
Mice
Reactive Oxygen Species
Cytochrome b Group
Membrane Glycoproteins
Membrane Proteins
Respiratory Burst
Phagocytosis
Immunity, Innate
HEK293 Cells
NADPH Oxidase 2
NADPH Oxidases
Research team
Functional Proteomics Group
Language
eng
Date accepted
2017-01-20
License start date
2017-04
Citation
The Journal of experimental medicine, 2017, 214 (4), pp. 1111 - 1128
Publisher
ROCKEFELLER UNIV PRESS