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Assessing HER2 Amplification in Plasma cfDNA.

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Date
2018-01
ICR Author
Garcia-Murillas, Isaac
Turner, Nicholas
Author
Garcia-Murillas, I
Turner, NC
Type
Journal Article
Metadata
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Abstract
Digital PCR (dPCR) is a highly accurate method to determine DNA concentration. In dPCR, DNA is portioned into many discrete single entities, and these are analyzed individually for the presence or absence of a target molecule of interest. Here we describe how digital PCR can be employed to determine the presence of oncogenic amplification through noninvasive analysis of circulating free DNA (cfDNA), and exemplify this approach by developing a plasma circulating free DNA dPCR assay for HER2 copy number.
URI
https://repository.icr.ac.uk/handle/internal/3101
DOI
https://doi.org/10.1007/978-1-4939-7778-9_10
Collections
  • Breast Cancer Research
Subject
Humans
Neoplasms
Receptor, erbB-2
Polymerase Chain Reaction
Gene Amplification
Gene Dosage
Circulating Tumor DNA
Research team
Molecular Oncology
Language
eng
Date accepted
2018-05-02
License start date
2018-01
Citation
Methods in molecular biology (Clifton, N.J.), 2018, 1768 pp. 161 - 172

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