Focused Ultrasound-Mediated Hyperthermia in Vitro: An Experimental Arrangement for Treating Cells under Tissue-Mimicking Conditions.
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Date
2019-12-01Author
Brüningk, SC
Rivens, I
Mouratidis, P
Ter Haar, G
Type
Journal Article
Metadata
Show full item recordAbstract
An experimental arrangement that allows in vitro exposure of cells to focused ultrasound-mediated hyperthermia (43°C-55°C) in a tissue-mimicking phantom with biological, acoustic and thermal properties comparable to those of human soft tissue is described. Cells were embedded in a compressed collagen gel, which was sandwiched between 6-mm-thick slices of biocompatible, acoustically absorbing and thermally tissue mimicking poly(vinyl alcohol) cryo-gel. To illustrate the system's potential, cells were exposed using a 1.66-MHz focused ultrasound beam (spatial-peak temporal-average intensities (ISPTA) = 900-1400 W/cm2) that traced out a circular trajectory (5-8 mm in diameter). Real-time temperature monitoring allowed cells to be exposed reproducibly to a pre-determined thermal dose. An experimental planning tool that estimates the thermal dose distribution throughout the sample and allows spatial correlation with cell position has been developed. Treatment response was evaluated qualitatively using microscopy and cell viability testing. This experimental arrangement has significant potential for future, biologically relevant, in vitro focused ultrasound-mediated hyperthermia studies.
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Subject
Hyperthermia, Induced
Phantoms, Imaging
High-Intensity Focused Ultrasound Ablation
In Vitro Techniques
Research team
Radiotherapy Physics Modelling
Therapeutic Ultrasound
Language
eng
Date accepted
2019-06-18
License start date
2019-12
Citation
Ultrasound in medicine & biology, 2019, 45 (12), pp. 3290 - 3297
Publisher
ELSEVIER SCIENCE INC