dc.contributor.author | Wamaitha, SE | |
dc.contributor.author | Grybel, KJ | |
dc.contributor.author | Alanis-Lobato, G | |
dc.contributor.author | Gerri, C | |
dc.contributor.author | Ogushi, S | |
dc.contributor.author | McCarthy, A | |
dc.contributor.author | Mahadevaiah, SK | |
dc.contributor.author | Healy, L | |
dc.contributor.author | Lea, RA | |
dc.contributor.author | Molina-Arcas, M | |
dc.contributor.author | Devito, LG | |
dc.contributor.author | Elder, K | |
dc.contributor.author | Snell, P | |
dc.contributor.author | Christie, L | |
dc.contributor.author | Downward, J | |
dc.contributor.author | Turner, JMA | |
dc.contributor.author | Niakan, KK | |
dc.date.accessioned | 2020-03-04T10:28:41Z | |
dc.date.issued | 2020-02-07 | |
dc.identifier.citation | Nature communications, 2020, 11 (1), pp. 764 - ? | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/3526 | |
dc.identifier.eissn | 2041-1723 | |
dc.identifier.doi | 10.1038/s41467-020-14629-x | |
dc.description.abstract | Our understanding of the signalling pathways regulating early human development is limited, despite their fundamental biological importance. Here, we mine transcriptomics datasets to investigate signalling in the human embryo and identify expression for the insulin and insulin growth factor 1 (IGF1) receptors, along with IGF1 ligand. Consequently, we generate a minimal chemically-defined culture medium in which IGF1 together with Activin maintain self-renewal in the absence of fibroblast growth factor (FGF) signalling. Under these conditions, we derive several pluripotent stem cell lines that express pluripotency-associated genes, retain high viability and a normal karyotype, and can be genetically modified or differentiated into multiple cell lineages. We also identify active phosphoinositide 3-kinase (PI3K)/AKT/mTOR signalling in early human embryos, and in both primed and naïve pluripotent culture conditions. This demonstrates that signalling insights from human blastocysts can be used to define culture conditions that more closely recapitulate the embryonic niche. | |
dc.format | Electronic | |
dc.format.extent | 764 - ? | |
dc.language | eng | |
dc.language.iso | eng | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Cells, Cultured | |
dc.subject | Fibroblasts | |
dc.subject | Blastocyst | |
dc.subject | Endoderm | |
dc.subject | Animals | |
dc.subject | Humans | |
dc.subject | Mice | |
dc.subject | Activins | |
dc.subject | Receptor, IGF Type 1 | |
dc.subject | Insulin-Like Growth Factor I | |
dc.subject | Culture Media | |
dc.subject | Coculture Techniques | |
dc.subject | Signal Transduction | |
dc.subject | Cell Differentiation | |
dc.subject | Gene Expression Regulation, Developmental | |
dc.subject | Extraembryonic Membranes | |
dc.subject | X Chromosome Inactivation | |
dc.subject | Induced Pluripotent Stem Cells | |
dc.subject | Phosphatidylinositol 3-Kinases | |
dc.subject | TOR Serine-Threonine Kinases | |
dc.subject | Transcriptome | |
dc.subject | Human Embryonic Stem Cells | |
dc.subject | Cell Self Renewal | |
dc.title | IGF1-mediated human embryonic stem cell self-renewal recapitulates the embryonic niche. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2020-01-23 | |
rioxxterms.versionofrecord | 10.1038/s41467-020-14629-x | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2020-02-07 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Nature communications | |
pubs.issue | 1 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Lung Cancer Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Lung Cancer Group | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Lung Cancer Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Lung Cancer Group | |
pubs.publication-status | Published | |
pubs.volume | 11 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Lung Cancer Group | |
dc.contributor.icrauthor | Downward, Julian David Harry | |