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dc.contributor.authorWamaitha, SE
dc.contributor.authorGrybel, KJ
dc.contributor.authorAlanis-Lobato, G
dc.contributor.authorGerri, C
dc.contributor.authorOgushi, S
dc.contributor.authorMcCarthy, A
dc.contributor.authorMahadevaiah, SK
dc.contributor.authorHealy, L
dc.contributor.authorLea, RA
dc.contributor.authorMolina-Arcas, M
dc.contributor.authorDevito, LG
dc.contributor.authorElder, K
dc.contributor.authorSnell, P
dc.contributor.authorChristie, L
dc.contributor.authorDownward, J
dc.contributor.authorTurner, JMA
dc.contributor.authorNiakan, KK
dc.date.accessioned2020-03-04T10:28:41Z
dc.date.issued2020-02-07
dc.identifier.citationNature communications, 2020, 11 (1), pp. 764 - ?
dc.identifier.issn2041-1723
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3526
dc.identifier.eissn2041-1723
dc.identifier.doi10.1038/s41467-020-14629-x
dc.description.abstractOur understanding of the signalling pathways regulating early human development is limited, despite their fundamental biological importance. Here, we mine transcriptomics datasets to investigate signalling in the human embryo and identify expression for the insulin and insulin growth factor 1 (IGF1) receptors, along with IGF1 ligand. Consequently, we generate a minimal chemically-defined culture medium in which IGF1 together with Activin maintain self-renewal in the absence of fibroblast growth factor (FGF) signalling. Under these conditions, we derive several pluripotent stem cell lines that express pluripotency-associated genes, retain high viability and a normal karyotype, and can be genetically modified or differentiated into multiple cell lineages. We also identify active phosphoinositide 3-kinase (PI3K)/AKT/mTOR signalling in early human embryos, and in both primed and naïve pluripotent culture conditions. This demonstrates that signalling insights from human blastocysts can be used to define culture conditions that more closely recapitulate the embryonic niche.
dc.formatElectronic
dc.format.extent764 - ?
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectCells, Cultured
dc.subjectFibroblasts
dc.subjectBlastocyst
dc.subjectEndoderm
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectActivins
dc.subjectReceptor, IGF Type 1
dc.subjectInsulin-Like Growth Factor I
dc.subjectCulture Media
dc.subjectCoculture Techniques
dc.subjectSignal Transduction
dc.subjectCell Differentiation
dc.subjectGene Expression Regulation, Developmental
dc.subjectExtraembryonic Membranes
dc.subjectX Chromosome Inactivation
dc.subjectInduced Pluripotent Stem Cells
dc.subjectPhosphatidylinositol 3-Kinases
dc.subjectTOR Serine-Threonine Kinases
dc.subjectTranscriptome
dc.subjectHuman Embryonic Stem Cells
dc.subjectCell Self Renewal
dc.titleIGF1-mediated human embryonic stem cell self-renewal recapitulates the embryonic niche.
dc.typeJournal Article
dcterms.dateAccepted2020-01-23
rioxxterms.versionofrecord10.1038/s41467-020-14629-x
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2020-02-07
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfNature communications
pubs.issue1
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Lung Cancer Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Lung Cancer Group
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Lung Cancer Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Lung Cancer Group
pubs.publication-statusPublished
pubs.volume11
pubs.embargo.termsNot known
icr.researchteamLung Cancer Groupen_US
dc.contributor.icrauthorDownward, Julian David Harry


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