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dc.contributor.authorWamaitha, SEen_US
dc.contributor.authorGrybel, KJen_US
dc.contributor.authorAlanis-Lobato, Gen_US
dc.contributor.authorGerri, Cen_US
dc.contributor.authorOgushi, Sen_US
dc.contributor.authorMcCarthy, Aen_US
dc.contributor.authorMahadevaiah, SKen_US
dc.contributor.authorHealy, Len_US
dc.contributor.authorLea, RAen_US
dc.contributor.authorMolina-Arcas, Men_US
dc.contributor.authorDevito, LGen_US
dc.contributor.authorElder, Ken_US
dc.contributor.authorSnell, Pen_US
dc.contributor.authorChristie, Len_US
dc.contributor.authorDownward, Jen_US
dc.contributor.authorTurner, JMAen_US
dc.contributor.authorNiakan, KKen_US
dc.date.accessioned2020-03-04T10:28:41Z
dc.date.issued2020-02-07en_US
dc.identifier.citationNature communications, 2020, 11 (1), pp. 764 - ?en_US
dc.identifier.issn2041-1723en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3526
dc.identifier.eissn2041-1723en_US
dc.identifier.doi10.1038/s41467-020-14629-xen_US
dc.description.abstractOur understanding of the signalling pathways regulating early human development is limited, despite their fundamental biological importance. Here, we mine transcriptomics datasets to investigate signalling in the human embryo and identify expression for the insulin and insulin growth factor 1 (IGF1) receptors, along with IGF1 ligand. Consequently, we generate a minimal chemically-defined culture medium in which IGF1 together with Activin maintain self-renewal in the absence of fibroblast growth factor (FGF) signalling. Under these conditions, we derive several pluripotent stem cell lines that express pluripotency-associated genes, retain high viability and a normal karyotype, and can be genetically modified or differentiated into multiple cell lineages. We also identify active phosphoinositide 3-kinase (PI3K)/AKT/mTOR signalling in early human embryos, and in both primed and naïve pluripotent culture conditions. This demonstrates that signalling insights from human blastocysts can be used to define culture conditions that more closely recapitulate the embryonic niche.en_US
dc.formatElectronicen_US
dc.format.extent764 - ?en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectCells, Cultureden_US
dc.subjectFibroblastsen_US
dc.subjectBlastocysten_US
dc.subjectEndodermen_US
dc.subjectAnimalsen_US
dc.subjectHumansen_US
dc.subjectMiceen_US
dc.subjectActivinsen_US
dc.subjectReceptor, IGF Type 1en_US
dc.subjectInsulin-Like Growth Factor Ien_US
dc.subjectCulture Mediaen_US
dc.subjectCoculture Techniquesen_US
dc.subjectSignal Transductionen_US
dc.subjectCell Differentiationen_US
dc.subjectGene Expression Regulation, Developmentalen_US
dc.subjectExtraembryonic Membranesen_US
dc.subjectX Chromosome Inactivationen_US
dc.subjectInduced Pluripotent Stem Cellsen_US
dc.subjectPhosphatidylinositol 3-Kinasesen_US
dc.subjectTOR Serine-Threonine Kinasesen_US
dc.subjectTranscriptomeen_US
dc.subjectHuman Embryonic Stem Cellsen_US
dc.subjectCell Self Renewalen_US
dc.titleIGF1-mediated human embryonic stem cell self-renewal recapitulates the embryonic niche.en_US
dc.typeJournal Article
dcterms.dateAccepted2020-01-23en_US
rioxxterms.versionofrecord10.1038/s41467-020-14629-xen_US
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0en_US
rioxxterms.licenseref.startdate2020-02-07en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfNature communicationsen_US
pubs.issue1en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Lung Cancer Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Lung Cancer Group
pubs.publication-statusPublisheden_US
pubs.volume11en_US
pubs.embargo.termsNot knownen_US
icr.researchteamLung Cancer Groupen_US
dc.contributor.icrauthorDownward, Julian David Harryen_US


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