dc.contributor.author | Cano-Gamez, E | |
dc.contributor.author | Soskic, B | |
dc.contributor.author | Roumeliotis, TI | |
dc.contributor.author | So, E | |
dc.contributor.author | Smyth, DJ | |
dc.contributor.author | Baldrighi, M | |
dc.contributor.author | Willé, D | |
dc.contributor.author | Nakic, N | |
dc.contributor.author | Esparza-Gordillo, J | |
dc.contributor.author | Larminie, CGC | |
dc.contributor.author | Bronson, PG | |
dc.contributor.author | Tough, DF | |
dc.contributor.author | Rowan, WC | |
dc.contributor.author | Choudhary, JS | |
dc.contributor.author | Trynka, G | |
dc.date.accessioned | 2020-05-26T11:08:48Z | |
dc.date.issued | 2020-04-14 | |
dc.identifier.citation | Nature communications, 2020, 11 (1), pp. 1801 - ? | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/3632 | |
dc.identifier.eissn | 2041-1723 | |
dc.identifier.doi | 10.1038/s41467-020-15543-y | |
dc.description.abstract | Naïve CD4+ T cells coordinate the immune response by acquiring an effector phenotype in response to cytokines. However, the cytokine responses in memory T cells remain largely understudied. Here we use quantitative proteomics, bulk RNA-seq, and single-cell RNA-seq of over 40,000 human naïve and memory CD4+ T cells to show that responses to cytokines differ substantially between these cell types. Memory T cells are unable to differentiate into the Th2 phenotype, and acquire a Th17-like phenotype in response to iTreg polarization. Single-cell analyses show that T cells constitute a transcriptional continuum that progresses from naïve to central and effector memory T cells, forming an effectorness gradient accompanied by an increase in the expression of chemokines and cytokines. Finally, we show that T cell activation and cytokine responses are influenced by the effectorness gradient. Our results illustrate the heterogeneity of T cell responses, furthering our understanding of inflammation. | |
dc.format | Electronic | |
dc.format.extent | 1801 - ? | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | NATURE RESEARCH | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | CD4-Positive T-Lymphocytes | |
dc.subject | Humans | |
dc.subject | Receptors, Antigen, T-Cell | |
dc.subject | Proteome | |
dc.subject | Cytokines | |
dc.subject | Lymphocyte Activation | |
dc.subject | Cell Polarity | |
dc.subject | Gene Expression Regulation | |
dc.subject | Principal Component Analysis | |
dc.subject | Middle Aged | |
dc.subject | Male | |
dc.subject | Single-Cell Analysis | |
dc.subject | Transcriptome | |
dc.subject | CD28 Antigens | |
dc.title | Single-cell transcriptomics identifies an effectorness gradient shaping the response of CD4+ T cells to cytokines. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2020-03-18 | |
rioxxterms.versionofrecord | 10.1038/s41467-020-15543-y | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2020-04-14 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Nature communications | |
pubs.issue | 1 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR | |
pubs.publication-status | Published | |
pubs.volume | 11 | |
pubs.embargo.terms | Not known | |
dc.contributor.icrauthor | Roumeliotis, Theodoros | |
dc.contributor.icrauthor | Choudhary, Jyoti | |