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dc.contributor.authorGrundy, GJ
dc.contributor.authorPolo, LM
dc.contributor.authorZeng, Z
dc.contributor.authorRulten, SL
dc.contributor.authorHoch, NC
dc.contributor.authorPaomephan, P
dc.contributor.authorXu, Y
dc.contributor.authorSweet, SM
dc.contributor.authorThorne, AW
dc.contributor.authorOliver, AW
dc.contributor.authorMatthews, SJ
dc.contributor.authorPearl, LH
dc.contributor.authorCaldecott, KW
dc.date.accessioned2020-06-17T08:53:02Z
dc.date.issued2016-08-17
dc.identifier.citationNature communications, 2016, 7 pp. 12404 - ?
dc.identifier.issn2041-1723
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3747
dc.identifier.eissn2041-1723
dc.identifier.doi10.1038/ncomms12404
dc.description.abstractPARP3 is a member of the ADP-ribosyl transferase superfamily that we show accelerates the repair of chromosomal DNA single-strand breaks in avian DT40 cells. Two-dimensional nuclear magnetic resonance experiments reveal that PARP3 employs a conserved DNA-binding interface to detect and stably bind DNA breaks and to accumulate at sites of chromosome damage. PARP3 preferentially binds to and is activated by mononucleosomes containing nicked DNA and which target PARP3 trans-ribosylation activity to a single-histone substrate. Although nicks in naked DNA stimulate PARP3 autoribosylation, nicks in mononucleosomes promote the trans-ribosylation of histone H2B specifically at Glu2. These data identify PARP3 as a molecular sensor of nicked nucleosomes and demonstrate, for the first time, the ribosylation of chromatin at a site-specific DNA single-strand break.
dc.formatElectronic
dc.format.extent12404 - ?
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectCell Line
dc.subjectChromosomes
dc.subjectChromatin
dc.subjectNucleosomes
dc.subjectAnimals
dc.subjectChickens
dc.subjectHumans
dc.subjectPoly(ADP-ribose) Polymerases
dc.subjectRibose
dc.subjectHistones
dc.subjectDNA
dc.subjectDNA Repair
dc.subjectModels, Molecular
dc.subjectDNA Breaks, Single-Stranded
dc.subjectProtein Domains
dc.titlePARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2B(Glu2).
dc.typeJournal Article
dcterms.dateAccepted2016-06-29
rioxxterms.versionofrecord10.1038/ncomms12404
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2016-08-17
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfNature communications
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR
pubs.publication-statusPublished
pubs.volume7
pubs.embargo.termsNot known
dc.contributor.icrauthorPearl, Laurenceen


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