dc.contributor.author | Krasny, L | |
dc.contributor.author | Bland, P | |
dc.contributor.author | Burns, J | |
dc.contributor.author | Lima, NC | |
dc.contributor.author | Harrison, PT | |
dc.contributor.author | Pacini, L | |
dc.contributor.author | Elms, ML | |
dc.contributor.author | Ning, J | |
dc.contributor.author | Martinez, VG | |
dc.contributor.author | Yu, Y-R | |
dc.contributor.author | Acton, SE | |
dc.contributor.author | Ho, P-C | |
dc.contributor.author | Calvo, F | |
dc.contributor.author | Swain, A | |
dc.contributor.author | Howard, BA | |
dc.contributor.author | Natrajan, RC | |
dc.contributor.author | Huang, PH | |
dc.date.accessioned | 2020-07-06T14:41:20Z | |
dc.date.issued | 2020-06-03 | |
dc.identifier.citation | Disease models & mechanisms, 2020, 13 (7) | |
dc.identifier.issn | 1754-8403 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/3815 | |
dc.identifier.eissn | 1754-8411 | |
dc.identifier.doi | 10.1242/dmm.044586 | |
dc.description.abstract | SWATH-mass spectrometry (MS) enables accurate and reproducible proteomic profiling in multiple model organisms including the mouse. Here, we present a comprehensive mouse reference spectral library (MouseRefSWATH) that permits quantification of up to 10,597 proteins (62.2% of the mouse proteome) by SWATH-MS. We exploit MouseRefSWATH to develop an analytical pipeline for species-specific deconvolution of proteomic alterations in human tumour xenografts (XenoSWATH). This method overcomes the challenge of high sequence similarity between mouse and human proteins, facilitating the study of host microenvironment-tumour interactions from 'bulk tumour' measurements. We apply the XenoSWATH pipeline to characterize an intraductal xenograft model of breast ductal carcinoma in situ and uncover complex regulation consistent with stromal reprogramming, where the modulation of cell migration pathways is not restricted to tumour cells but also operates in the mouse stroma upon progression to invasive disease. MouseRefSWATH and XenoSWATH open new opportunities for in-depth and reproducible proteomic assessment to address wide-ranging biological questions involving this important model organism. | |
dc.format | Electronic | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | COMPANY BIOLOGISTS LTD | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.title | A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2020-05-20 | |
rioxxterms.versionofrecord | 10.1242/dmm.044586 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2020-07-14 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Disease models & mechanisms | |
pubs.issue | 7 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research/Endocrine control mechanisms | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research/Functional Genomics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Development & Cancer | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Development & Cancer | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Functional Genomics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Molecular and Systems Oncology | |
pubs.organisational-group | /ICR/Students | |
pubs.organisational-group | /ICR/Students/PhD and MPhil | |
pubs.organisational-group | /ICR/Students/PhD and MPhil/16/17 Starting Cohort | |
pubs.organisational-group | /ICR/Students/PhD and MPhil/17/18 Starting Cohort | |
pubs.organisational-group | /ICR/Students/PhD and MPhil/18/19 Starting Cohort | |
pubs.publication-status | Published | |
pubs.volume | 13 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Endocrine control mechanisms | |
icr.researchteam | Development & Cancer | |
icr.researchteam | Functional Genomics | |
icr.researchteam | Molecular and Systems Oncology | |
dc.contributor.icrauthor | Krasny, Lukas | |
dc.contributor.icrauthor | Burns, Jessica | |
dc.contributor.icrauthor | Harrison, Peter | |
dc.contributor.icrauthor | Elms, Mark | |
dc.contributor.icrauthor | Swain, Amanda | |
dc.contributor.icrauthor | Howard, Beatrice | |
dc.contributor.icrauthor | Natrajan, Rachael | |
dc.contributor.icrauthor | Huang, Paul | |