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dc.contributor.authorKrasny, Len_US
dc.contributor.authorBland, Pen_US
dc.contributor.authorBurns, Jen_US
dc.contributor.authorLima, NCen_US
dc.contributor.authorHarrison, PTen_US
dc.contributor.authorPacini, Len_US
dc.contributor.authorElms, MLen_US
dc.contributor.authorNing, Jen_US
dc.contributor.authorMartinez, VGen_US
dc.contributor.authorYu, Y-Ren_US
dc.contributor.authorActon, SEen_US
dc.contributor.authorHo, P-Cen_US
dc.contributor.authorCalvo, Fen_US
dc.contributor.authorSwain, Aen_US
dc.contributor.authorHoward, BAen_US
dc.contributor.authorNatrajan, RCen_US
dc.contributor.authorHuang, PHen_US
dc.date.accessioned2020-07-06T14:41:20Z
dc.date.issued2020-07-14
dc.identifier.citationDisease models & mechanisms, 2020, 13 (7)
dc.identifier.issn1754-8403
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3815
dc.identifier.eissn1754-8411
dc.identifier.doi10.1242/dmm.044586
dc.description.abstractSWATH-mass spectrometry (MS) enables accurate and reproducible proteomic profiling in multiple model organisms including the mouse. Here, we present a comprehensive mouse reference spectral library (MouseRefSWATH) that permits quantification of up to 10,597 proteins (62.2% of the mouse proteome) by SWATH-MS. We exploit MouseRefSWATH to develop an analytical pipeline for species-specific deconvolution of proteomic alterations in human tumour xenografts (XenoSWATH). This method overcomes the challenge of high sequence similarity between mouse and human proteins, facilitating the study of host microenvironment-tumour interactions from 'bulk tumour' measurements. We apply the XenoSWATH pipeline to characterize an intraductal xenograft model of breast ductal carcinoma in situ and uncover complex regulation consistent with stromal reprogramming, where the modulation of cell migration pathways is not restricted to tumour cells but also operates in the mouse stroma upon progression to invasive disease. MouseRefSWATH and XenoSWATH open new opportunities for in-depth and reproducible proteomic assessment to address wide-ranging biological questions involving this important model organism.
dc.formatElectronic
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleA mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts.
dc.typeJournal Article
dcterms.dateAccepted2020-05-20
rioxxterms.versionofrecord10.1242/dmm.044586
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2020-07-14
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfDisease models & mechanisms
pubs.issue7
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research/Endocrine control mechanisms
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research/Functional Genomics
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Development & Cancer
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Development & Cancer
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Functional Genomics
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Molecular and Systems Oncology
pubs.organisational-group/ICR/Students
pubs.organisational-group/ICR/Students/PhD and MPhil
pubs.organisational-group/ICR/Students/PhD and MPhil/16/17 Starting Cohort
pubs.organisational-group/ICR/Students/PhD and MPhil/17/18 Starting Cohort
pubs.organisational-group/ICR/Students/PhD and MPhil/18/19 Starting Cohort
pubs.publication-statusPublished
pubs.volume13
pubs.embargo.termsNot known
icr.researchteamEndocrine control mechanismsen_US
icr.researchteamDevelopment & Canceren_US
icr.researchteamFunctional Genomicsen_US
icr.researchteamMolecular and Systems Oncologyen_US
dc.contributor.icrauthorHuang, Paulen
dc.contributor.icrauthorElms, Marken
dc.contributor.icrauthorSwain, Amandaen
dc.contributor.icrauthorKrasny, Lukasen
dc.contributor.icrauthorHoward, Beatriceen
dc.contributor.icrauthorNatrajan, Rachaelen
dc.contributor.icrauthorHarrison, Peteren
dc.contributor.icrauthorBurns, Jessicaen


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Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0