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dc.contributor.authorBryant, SL
dc.contributor.authorFrancis, JC
dc.contributor.authorLokody, IB
dc.contributor.authorWang, H
dc.contributor.authorRisbridger, GP
dc.contributor.authorLoveland, KL
dc.contributor.authorSwain, A
dc.date.accessioned2020-08-06T14:55:22Z
dc.date.issued2014-11
dc.identifier.citationDevelopmental biology, 2014, 395 (2), pp. 209 - 217
dc.identifier.issn0012-1606
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3920
dc.identifier.eissn1095-564X
dc.identifier.doi10.1016/j.ydbio.2014.09.016
dc.description.abstractThe mammalian urogenital sinus (UGS) develops in a sex specific manner, giving rise to the prostate in the male and the sinus vagina in the embryonic female. Androgens, produced by the embryonic testis, have been shown to be crucial to this process. In this study we show that retinoic acid signaling is required for the initial stages of bud development from the male UGS. Enzymes involved in retinoic acid synthesis are expressed in the UGS mesenchyme in a sex specific manner and addition of ligand to female tissue is able to induce prostate-like bud formation in the absence of androgens, albeit at reduced potency. Functional studies in mouse organ cultures that faithfully reproduce the initiation of prostate development indicate that one of the roles of retinoic acid signaling in the male is to inhibit the expression of Inhba, which encodes the βA subunit of Activin, in the UGS mesenchyme. Through in vivo genetic analysis and culture studies we show that inhibition of Activin signaling in the female UGS leads to a similar phenotype to that of retinoic acid treatment, namely bud formation in the absence of androgens. Our data also reveals that both androgens and retinoic acid have extra independent roles to that of repressing Activin signaling in the development of the prostate during fetal stages. This study identifies a novel role for retinoic acid as a mesenchymal factor that acts together with androgens to determine the position and initiation of bud development in the male UGS epithelia.
dc.formatPrint-Electronic
dc.format.extent209 - 217
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectUrogenital System
dc.subjectProstate
dc.subjectAnimals
dc.subjectMice
dc.subjectTretinoin
dc.subjectActivins
dc.subjectInhibin-beta Subunits
dc.subjectbeta-Galactosidase
dc.subjectDNA Primers
dc.subjectImmunohistochemistry
dc.subjectIn Situ Hybridization
dc.subjectSex Factors
dc.subjectSignal Transduction
dc.subjectOrganogenesis
dc.subjectFemale
dc.subjectMale
dc.subjectReal-Time Polymerase Chain Reaction
dc.titleSex specific retinoic acid signaling is required for the initiation of urogenital sinus bud development.
dc.typeJournal Article
dcterms.dateAccepted2014-09-17
rioxxterms.versionofrecord10.1016/j.ydbio.2014.09.016
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2014-11
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfDevelopmental biology
pubs.issue2
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Development & Cancer
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Development & Cancer
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Development & Cancer
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Development & Cancer
pubs.publication-statusPublished
pubs.volume395
pubs.embargo.termsNot known
icr.researchteamDevelopment & Canceren_US
dc.contributor.icrauthorBryant, Sarah Louiseen
dc.contributor.icrauthorSwain, Amandaen


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