DNA methylation of the long intergenic noncoding RNA 299 gene in triple-negative breast cancer: results from a prospective study.
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Date
2020-07-16Author
Manoochehri, M
Jones, M
Tomczyk, K
Fletcher, O
Schoemaker, MJ
Swerdlow, AJ
Borhani, N
Hamann, U
Type
Journal Article
Metadata
Show full item recordAbstract
Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype associated with a high rate of recurrence and poor prognosis. Recently we identified a hypermethylation in the long noncoding RNA 299 (LINC00299) gene in blood-derived DNA from TNBC patients compared with healthy controls implying that LINC00299 hypermethylation may serve as a circulating biomarker for TNBC. In the present study, we investigated whether LINC00299 methylation is associated with TNBC in a prospective nested breast cancer case-control study within the Generations Study. Methylation at cg06588802 in LINC00299 was measured in 154 TNBC cases and 159 breast cancer-free matched controls using MethyLight droplet digital PCR. To assess the association between methylation level and TNBC risk, logistic regression was used to calculate odd ratios and 95% confidence intervals, adjusted for smoking status. We found no evidence for association between methylation levels and TNBC overall (P = 0.062). Subgroup analysis according to age at diagnosis and age at blood draw revealed increased methylation levels in TNBC cases compared with controls in the young age groups [age 26-52 (P = 0.0025) and age 22-46 (P = 0.001), respectively]. Our results suggest a potential association of LINC00299 hypermethylation with TNBC in young women.
Collections
Subject
Cell Line, Tumor
Humans
Odds Ratio
Gene Expression Profiling
Age of Onset
DNA Methylation
Gene Expression Regulation, Neoplastic
Genetic Heterogeneity
Adult
Aged
Aged, 80 and over
Middle Aged
Female
RNA, Long Noncoding
Triple Negative Breast Neoplasms
Biomarkers, Tumor
Research team
Aetiological Epidemiology
Language
eng
Date accepted
2020-05-27
License start date
2020-07-16
Citation
Scientific reports, 2020, 10 (1), pp. 11762 - ?
Publisher
NATURE PUBLISHING GROUP