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dc.contributor.authorTedeschi, A
dc.contributor.authorAlmagro, J
dc.contributor.authorRenshaw, MJ
dc.contributor.authorMessal, HA
dc.contributor.authorBehrens, A
dc.contributor.authorPetronczki, M
dc.date.accessioned2020-08-27T12:18:29Z
dc.date.issued2020-04-08
dc.identifier.citationNature communications, 2020, 11 (1), pp. 1746 - ?
dc.identifier.issn2041-1723
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4042
dc.identifier.eissn2041-1723
dc.identifier.doi10.1038/s41467-020-15359-w
dc.description.abstractIn mammalian cell lines, the endosomal sorting complex required for transport (ESCRT)-III mediates abscission, the process that physically separates daughter cells and completes cell division. Cep55 protein is regarded as the master regulator of abscission, because it recruits ESCRT-III to the midbody (MB), the site of abscission. However, the importance of this mechanism in a mammalian organism has never been tested. Here we show that Cep55 is dispensable for mouse embryonic development and adult tissue homeostasis. Cep55-knockout offspring show microcephaly and primary neural progenitors require Cep55 and ESCRT for survival and abscission. However, Cep55 is dispensable for cell division in embryonic or adult tissues. In vitro, division of primary fibroblasts occurs without Cep55 and ESCRT-III at the midbody and is not affected by ESCRT depletion. Our work defines Cep55 as an abscission regulator only in specific tissue contexts and necessitates the re-evaluation of an alternative ESCRT-independent cell division mechanism.
dc.formatElectronic
dc.format.extent1746 - ?
dc.languageeng
dc.language.isoeng
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectKidney
dc.subjectCerebral Cortex
dc.subjectCells, Cultured
dc.subjectFibroblasts
dc.subjectAnimals
dc.subjectMice, Inbred C57BL
dc.subjectAnimals, Newborn
dc.subjectMice, Knockout
dc.subjectMicrocephaly
dc.subjectCell Cycle Proteins
dc.subjectCytokinesis
dc.subjectMitosis
dc.subjectApoptosis
dc.subjectGene Deletion
dc.subjectGenotype
dc.subjectEndosomal Sorting Complexes Required for Transport
dc.subjectNeural Stem Cells
dc.titleCep55 promotes cytokinesis of neural progenitors but is dispensable for most mammalian cell divisions.
dc.typeJournal Article
dcterms.dateAccepted2020-02-27
rioxxterms.versionofrecord10.1038/s41467-020-15359-w
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2020-04-08
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfNature communications
pubs.issue1
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR
pubs.publication-statusPublished
pubs.volume11
pubs.embargo.termsNot known
dc.contributor.icrauthorBehrens, Axel


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