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dc.contributor.authorGreber, BJ
dc.contributor.authorToso, DB
dc.contributor.authorFang, J
dc.contributor.authorNogales, E
dc.date.accessioned2020-09-16T13:22:52Z
dc.date.issued2019-03-12
dc.identifier.citationeLife, 2019, 8
dc.identifier.issn2050-084X
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4068
dc.identifier.eissn2050-084Xen_US
dc.identifier.doi10.7554/elife.44771en_US
dc.description.abstractTranscription factor IIH (TFIIH) is a heterodecameric protein complex critical for transcription initiation by RNA polymerase II and nucleotide excision DNA repair. The TFIIH core complex is sufficient for its repair functions and harbors the XPB and XPD DNA-dependent ATPase/helicase subunits, which are affected by human disease mutations. Transcription initiation additionally requires the CdK activating kinase subcomplex. Previous structural work has provided only partial insight into the architecture of TFIIH and its interactions within transcription pre-initiation complexes. Here, we present the complete structure of the human TFIIH core complex, determined by phase-plate cryo-electron microscopy at 3.7 Å resolution. The structure uncovers the molecular basis of TFIIH assembly, revealing how the recruitment of XPB by p52 depends on a pseudo-symmetric dimer of homologous domains in these two proteins. The structure also suggests a function for p62 in the regulation of XPD, and allows the mapping of previously unresolved human disease mutations.
dc.formatElectronic
dc.languageeng
dc.language.isoeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHela Cells
dc.subjectHumans
dc.subjectDNA Damage
dc.subjectDNA Helicases
dc.subjectRNA-Binding Proteins
dc.subjectCell Cycle Proteins
dc.subjectDNA-Binding Proteins
dc.subjectTranscription Factors
dc.subjectCryoelectron Microscopy
dc.subjectDNA Repair
dc.subjectTranscription, Genetic
dc.subjectProtein Conformation
dc.subjectProtein Binding
dc.subjectMutation
dc.subjectTranscription Factor TFIIH
dc.subjectXeroderma Pigmentosum Group D Protein
dc.subjectNF-kappa B p52 Subunit
dc.subjectProtein Domains
dc.titleThe complete structure of the human TFIIH core complex.
dc.typeJournal Article
dcterms.dateAccepted2019-03-03
rioxxterms.versionofrecord10.7554/elife.44771
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2019-03-12en_US
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfeLife
pubs.notesNo embargo
pubs.organisational-group/ICR
pubs.organisational-group/ICR
pubs.publication-statusPublished
pubs.volume8en_US
pubs.embargo.termsNo embargo
dc.contributor.icrauthorGreber, Basilen


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/