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dc.contributor.authorInvergo, BM
dc.contributor.authorBrochet, M
dc.contributor.authorYu, L
dc.contributor.authorChoudhary, J
dc.contributor.authorBeltrao, P
dc.contributor.authorBillker, O
dc.date.accessioned2020-10-15T15:24:03Z
dc.date.issued2017-11
dc.identifier.citationCell reports, 2017, 21 (7), pp. 2017 - 2029
dc.identifier.issn2211-1247
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4157
dc.identifier.eissn2211-1247
dc.identifier.doi10.1016/j.celrep.2017.10.071
dc.description.abstractThe transmission of malaria parasites to mosquitoes relies on the rapid induction of sexual reproduction upon their ingestion into a blood meal. Haploid female and male gametocytes become activated and emerge from their host cells, and the males enter the cell cycle to produce eight microgametes. The synchronized nature of gametogenesis allowed us to investigate phosphorylation signaling during its first minute in Plasmodium berghei via a high-resolution time course of the phosphoproteome. This revealed an unexpectedly broad response, with proteins related to distinct cell cycle events undergoing simultaneous phosphoregulation. We implicate several protein kinases in the process, and we validate our analyses on the plant-like calcium-dependent protein kinase 4 (CDPK4) and a homolog of serine/arginine-rich protein kinases (SRPK1). Mutants in these kinases displayed distinct phosphoproteomic disruptions, consistent with differences in their phenotypes. The results reveal the central role of protein phosphorylation in the atypical cell cycle regulation of a divergent eukaryote.
dc.formatPrint
dc.format.extent2017 - 2029
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectAnimals
dc.subjectMice
dc.subjectPlasmodium berghei
dc.subjectProtein-Serine-Threonine Kinases
dc.subjectProtozoan Proteins
dc.subjectSignal Transduction
dc.subjectCell Cycle
dc.subjectGametogenesis
dc.subjectProtein Processing, Post-Translational
dc.subjectPhosphorylation
dc.subjectLife Cycle Stages
dc.subjectFemale
dc.subjectMale
dc.titleSub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium Gamete Formation.
dc.typeJournal Article
dcterms.dateAccepted2017-10-18
rioxxterms.versionofrecord10.1016/j.celrep.2017.10.071
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2017-11
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfCell reports
pubs.issue7
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR
pubs.publication-statusPublished
pubs.volume21
pubs.embargo.termsNot known
dc.contributor.icrauthorChoudhary, Jyotien


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