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dc.contributor.authorScanlan, Een_US
dc.contributor.authorYu, Len_US
dc.contributor.authorMaskell, Den_US
dc.contributor.authorChoudhary, Jen_US
dc.contributor.authorGrant, Aen_US
dc.date.accessioned2020-10-16T09:23:01Z
dc.date.issued2017-01en_US
dc.identifier.citationJournal of proteomics, 2017, 152 pp. 181 - 187en_US
dc.identifier.issn1874-3919en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4169
dc.identifier.eissn1876-7737en_US
dc.identifier.doi10.1016/j.jprot.2016.11.009en_US
dc.description.abstractCampylobacter jejuni is the leading cause of bacterial gastroenteritis in the world. A number of factors are believed to contribute to the ability of C. jejuni to cause disease within the human host including the secretion of non-flagellar proteins via the flagellar type III secretion system (FT3SS). Here for the first time we have utilised quantitative proteomics using stable isotope labelling by amino acids in cell culture (SILAC), and label-free liquid chromatography-mass spectrometry (LC/MS), to compare supernatant samples from C. jejuni M1 wild type and flagella-deficient (flgG mutant) strains to identify putative novel proteins secreted via the FT3SS. Genes encoding proteins that were candidates for flagellar secretion, derived from the LC/MS and SILAC datasets, were deleted. Infection of human CACO-2 tissue culture cells using these mutants resulted in the identification of novel genes required for interactions with these cells. This work has shown for the first time that both CJM1_0791 and CJM1_0395 are dependent on the flagellum for their presence in supernatants from C. jejuni stains M1 and 81-176.This study provides the most complete description of the Campylobac er jejuni secretome to date. SILAC and label-free proteomics comparing mutants with or without flagella have resulted in the identification of two C. jejuni proteins that are dependent on flagella for their export from the bacterial cell.en_US
dc.formatPrint-Electronicen_US
dc.format.extent181 - 187en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectCaco-2 Cellsen_US
dc.subjectFlagellaen_US
dc.subjectHumansen_US
dc.subjectCampylobacter jejunien_US
dc.subjectGastroenteritisen_US
dc.subjectBacterial Proteinsen_US
dc.subjectChromatography, Liquiden_US
dc.subjectIsotope Labelingen_US
dc.subjectProteomicsen_US
dc.subjectTandem Mass Spectrometryen_US
dc.subjectBacterial Secretion Systemsen_US
dc.subjectType III Secretion Systemsen_US
dc.titleA quantitative proteomic screen of the Campylobacter jejuni flagellar-dependent secretome.en_US
dc.typeJournal Article
dcterms.dateAccepted2016-11-14en_US
rioxxterms.versionofrecord10.1016/j.jprot.2016.11.009en_US
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0en_US
rioxxterms.licenseref.startdate2017-01en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfJournal of proteomicsen_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.publication-statusPublisheden_US
pubs.volume152en_US
pubs.embargo.termsNot knownen_US
dc.contributor.icrauthorChoudhary, Jyotien_US


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