dc.contributor.author | Modrzynska, K | |
dc.contributor.author | Pfander, C | |
dc.contributor.author | Chappell, L | |
dc.contributor.author | Yu, L | |
dc.contributor.author | Suarez, C | |
dc.contributor.author | Dundas, K | |
dc.contributor.author | Gomes, AR | |
dc.contributor.author | Goulding, D | |
dc.contributor.author | Rayner, JC | |
dc.contributor.author | Choudhary, J | |
dc.contributor.author | Billker, O | |
dc.date.accessioned | 2020-10-16T09:24:17Z | |
dc.date.issued | 2017-01-11 | |
dc.identifier.citation | Cell host & microbe, 2017, 21 (1), pp. 11 - 22 | |
dc.identifier.issn | 1931-3128 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/4170 | |
dc.identifier.eissn | 1934-6069 | |
dc.identifier.doi | 10.1016/j.chom.2016.12.003 | |
dc.description.abstract | A family of apicomplexa-specific proteins containing AP2 DNA-binding domains (ApiAP2s) was identified in malaria parasites. This family includes sequence-specific transcription factors that are key regulators of development. However, functions for the majority of ApiAP2 genes remain unknown. Here, a systematic knockout screen in Plasmodium berghei identified ten ApiAP2 genes that were essential for mosquito transmission: four were critical for the formation of infectious ookinetes, and three were required for sporogony. We describe non-essential functions for AP2-O and AP2-SP proteins in blood stages, and identify AP2-G2 as a repressor active in both asexual and sexual stages. Comparative transcriptomics across mutants and developmental stages revealed clusters of co-regulated genes with shared cis promoter elements, whose expression can be controlled positively or negatively by different ApiAP2 factors. We propose that stage-specific interactions between ApiAP2 proteins on partly overlapping sets of target genes generate the complex transcriptional network that controls the Plasmodium life cycle. | |
dc.format | Print | |
dc.format.extent | 11 - 22 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | CELL PRESS | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Animals | |
dc.subject | Mice | |
dc.subject | Anopheles | |
dc.subject | Apicomplexa | |
dc.subject | Plasmodium berghei | |
dc.subject | Oocysts | |
dc.subject | Malaria | |
dc.subject | DNA-Binding Proteins | |
dc.subject | Protozoan Proteins | |
dc.subject | Life Cycle Stages | |
dc.subject | Female | |
dc.subject | Gene Knockout Techniques | |
dc.subject | Protein Domains | |
dc.title | A Knockout Screen of ApiAP2 Genes Reveals Networks of Interacting Transcriptional Regulators Controlling the Plasmodium Life Cycle. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2016-11-17 | |
rioxxterms.versionofrecord | 10.1016/j.chom.2016.12.003 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2017-01 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Cell host & microbe | |
pubs.issue | 1 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR | |
pubs.publication-status | Published | |
pubs.volume | 21 | |
pubs.embargo.terms | Not known | |
dc.contributor.icrauthor | Choudhary, Jyoti | |