dc.contributor.author | Sparks, H | |
dc.contributor.author | Dent, L | |
dc.contributor.author | Bakal, C | |
dc.contributor.author | Behrens, A | |
dc.contributor.author | Salbreux, G | |
dc.contributor.author | Dunsby, C | |
dc.date.accessioned | 2021-01-27T16:27:21Z | |
dc.date.issued | 2020-12-01 | |
dc.identifier.citation | Biomedical optics express, 2020, 11 (12), pp. 7204 - 7220 | |
dc.identifier.issn | 2156-7085 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/4324 | |
dc.identifier.eissn | 2156-7085 | |
dc.identifier.doi | 10.1364/boe.409781 | |
dc.description.abstract | We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a water immersion 40× 1.15 NA primary objective, deconvolved image volumes of 200 nm beads were measured to have full width at half maxima (FWHM) of 0.35 ± 0.04 µm and 0.39 ± 0.02 µm laterally and 0.81 ± 0.07 µm axially. The measured z-sectioning value was 1.33 ± 0.45 µm using light-sheet FWHM in the frames of the two views of 4.99 ± 0.58 µm and 4.89 ± 0.63 µm. To qualitatively demonstrate that the system can reduce shadow artefacts while providing a more isotropic resolution, a multi-cellular spheroid approximately 100 µm in diameter was imaged. | |
dc.format | Electronic-eCollection | |
dc.format.extent | 7204 - 7220 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | OPTICAL SOC AMER | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.title | Dual-view oblique plane microscopy (dOPM). | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2020-10-28 | |
rioxxterms.versionofrecord | 10.1364/boe.409781 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2020-12 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Biomedical optics express | |
pubs.issue | 12 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.publication-status | Published | |
pubs.volume | 11 | |
pubs.embargo.terms | Not known | |
dc.contributor.icrauthor | Bakal, Christopher | |
dc.contributor.icrauthor | Behrens, Axel | |