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dc.contributor.authorAntoniadi, I
dc.contributor.authorNovák, O
dc.contributor.authorGelová, Z
dc.contributor.authorJohnson, A
dc.contributor.authorPlíhal, O
dc.contributor.authorSimerský, R
dc.contributor.authorMik, V
dc.contributor.authorVain, T
dc.contributor.authorMateo-Bonmatí, E
dc.contributor.authorKarady, M
dc.contributor.authorPernisová, M
dc.contributor.authorPlačková, L
dc.contributor.authorOpassathian, K
dc.contributor.authorHejátko, J
dc.contributor.authorRobert, S
dc.contributor.authorFriml, J
dc.contributor.authorDoležal, K
dc.contributor.authorLjung, K
dc.contributor.authorTurnbull, C
dc.contributor.authorTurnbull, C
dc.date.accessioned2021-04-21T10:56:26Z
dc.date.available2021-04-21T10:56:26Z
dc.date.issued2020-08-27
dc.identifier.citationNature communications, 2020, 11 (1), pp. 4284 - ?
dc.identifier.issn2041-1723
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4536
dc.identifier.eissn2041-1723
dc.identifier.doi10.1038/s41467-020-17700-9
dc.description.abstractCytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses.en_US
dc.description.abstractCytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn
dc.formatElectronic
dc.format.extent4284 - ?
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectExtracellular Fluiden_US
dc.subjectPlants, Genetically Modifieden_US
dc.subjectArabidopsisen_US
dc.subjectAdenineen_US
dc.subjectCytokininsen_US
dc.subjectGreen Fluorescent Proteinsen_US
dc.subjectArabidopsis Proteinsen_US
dc.subjectRecombinant Proteinsen_US
dc.subjectSignal Transductionen_US
dc.subjectMutationen_US
dc.subjectHistidine Kinaseen_US
dc.titleCell-surface receptors enable perception of extracellular cytokinins.
dc.typeJournal Article
dcterms.dateAccepted2020-07-14
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1038/s41467-020-17700-9
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2020-08-27
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfNature communicationsen_US
pubs.issue1
pubs.notesNot known
pubs.organisational-group/ICR
pubs.publication-statusPublished
pubs.volume11en_US
pubs.embargo.termsNot known
dc.contributor.icrauthorTurnbull, Clareen


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